Literature summary for 2.4.1.B79 extracted from

Ma, B.; Liu, X.; Lu, Y.; Ma, X.; Wu, X.; Wang, X.; Jia, M.; Su, P.; Tong, Y.; Guan, H.; Jiang, Z.; Gao, J.; Huang, L.; Gao, W.
A specific UDP-glucosyltransferase catalyzes the formation of triptophenolide glucoside from Tripterygium wilfordii Hook. f. (2019), Phytochemistry, 166, 112062 .

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Application

Application	Comment	Organism
medicine	product triptophenolide 14-O-beta-D-glucopyranoside exhibits significant inhibitory effects on U87-MG, U251, C6, MCF-7, HeLa, K562, and RBL-2H3 cells	Tripterygium wilfordii

Crystallization (Commentary)

Crystallization (Comment)	Organism
molecular docking of substrates. Residue H30 exhibits a hydrogen bond interaction with triptophenolide. Residues W369, A370, Q372, N391, S392, E395, and W396 interact with uridine diphosphate glucose and exhibit hydrogen bond interactions with uridine diphosphate glucose	Tripterygium wilfordii

Protein Variants

Protein Variants	Comment	Organism
additional information	site-directed mutagenesis of residues His30, Asp132, Phe134, Thr154, Ala370, Leu376, Gly382, His387, Glu395 and Gln412, none of the 11 mutants can catalyze the glycosylation of triptophenolide	Tripterygium wilfordii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0127	-	triptophenolide	pH 7.5, 30°C	Tripterygium wilfordii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	divalent cations are not required nor improve the activity	Tripterygium wilfordii

Organism

Organism	UniProt	Comment	Textmining
Tripterygium wilfordii	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-beta-D-glucose + 4-methylumbelliferone	-	Tripterygium wilfordii	UDP + 4-methylumbelliferyl-beta-D-glucopyranoside	-	?
UDP-beta-D-glucose + liquiritigenin	-	Tripterygium wilfordii	UDP + neoliquiritin	-	?
UDP-beta-D-glucose + phloretin	-	Tripterygium wilfordii	UDP + phlorizin	-	?
UDP-beta-D-glucose + pinocembrin	-	Tripterygium wilfordii	UDP + pinocembrin 7-O-beta-D-glucopyranoside	-	?
UDP-beta-D-glucose + rhapontigenin	-	Tripterygium wilfordii	UDP + rhapontin	-	?
UDP-beta-D-glucose + triptophenolide	-	Tripterygium wilfordii	UDP + triptophenolide 14-O-beta-D-glucopyranoside	-	?

Synonyms

Synonyms	Comment	Organism
UGT1	-	Tripterygium wilfordii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Tripterygium wilfordii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.07	-	triptophenolide	pH 7.5, 30°C	Tripterygium wilfordii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Tripterygium wilfordii

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Release 2023.1 (January 2023)