General Stability | Organism |
---|---|
chromatography on Biogel A-1.5M decreases stability | Homo sapiens |
extremely stable to freezing, -40°C, and thawing cycles, soluble enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | soluble enzyme, above 50 mM | Homo sapiens | |
additional information | membrane-bound enzyme: not inhibited by 100 mM D-glucosamine, 10 mM N-ethylmaleimide, 50 mM glucosylgalactosylhydroxylysine, soluble enzyme: not inhibited by 75 mM glucosylgalactosylhydroxylysine | Homo sapiens | |
p-chloromercuribenzoate | membrane-bound enzyme, 6 and 10 mM: 54% inhibition | Homo sapiens | |
sucrose | membrane-bound enzyme, 0.42 M: 88% inhibition, also inhibits soluble enzyme | Homo sapiens | |
UDP | competitive to UDP-glucose; membrane-bound enzyme, 6 mM: complete inhibition; non-competitive to galactosylhydroxylysine; product inhibition | Homo sapiens | |
UTP | membrane-bound enzyme, 6 mM: complete inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Homo sapiens | |
additional information | - |
additional information | Km: 1.5 mg/ml, alpha-chain of chicken skin collagen | Homo sapiens | |
0.002 | - |
D-galactosylhydroxylysine | bound in purified alpha1-chain of chicken skin collagen, value based on actual amount of galactosylhydroxylysine-residues of alpha1-chain | Homo sapiens | |
0.005 | - |
UDP-glucose | membrane-bound enzyme | Homo sapiens | |
0.029 | - |
TDP-glucose | soluble enzyme | Homo sapiens | |
3.8 | - |
D-galactosylhydroxylysine | membrane-bound enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | 5-10% of the total activity is surface-bound, the rest is of cytoplasmic origin | Homo sapiens | 5737 | - |
membrane | membrane-bound | Homo sapiens | 16020 | - |
plasma membrane | 5-10% of the total activity is surface-bound, the rest is of cytoplasmic origin | Homo sapiens | 5886 | - |
soluble | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 64% as effective as Mn2+ | Homo sapiens | |
Mg2+ | 14% as effective as Mn2+ | Homo sapiens | |
Mn2+ | requirement | Homo sapiens | |
Mn2+ | 25-80 mM: optimal concentration for free galactosylhydroxylysine as acceptor, 2 mM: optimal concentration for galactosylhydroxylysine bound in purified alpha1-chain of chicken skin collagen as acceptor, soluble enzyme is inhibited above 50 mM | Homo sapiens | |
Mn2+ | most effective metal cofactor | Homo sapiens | |
additional information | not activated by Ca2+ | Homo sapiens | |
additional information | not activated by Cd2+ | Homo sapiens | |
additional information | not activated by Zn2+, Cu2+ | Homo sapiens | |
Ni2+ | activates, soluble enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
80fold: soluble enzyme | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + [procollagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine = UDP + [procollagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine | reaction mechanism | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood platelet | intact platelets and isolated plasma membranes | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0019 | - |
purified soluble enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
-40°C, membrane-preparation, 1-2 months, stable | Homo sapiens |
-40°C, soluble enzyme after Biogel A-1.5M chromatography, 2-4 weeks, stable | Homo sapiens |
room temperature, soluble enzyme after DEAE-cellulose column, 18 h, 10% loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: GDPglucose | Homo sapiens | ? | - |
? | |
additional information | not: ADPglucose | Homo sapiens | ? | - |
? | |
TDP-glucose + D-galactosylhydroxylysine | soluble enzyme, glucosylation at 60% of the rate of UDP-glucose | Homo sapiens | TDP + 1,2-D-glucosyl-D-galactosylhydroxylysine | - |
? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | acetic acid-soluble bovine tendon collagen | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | product is glucosylgalactosylhydroxylysine-collagen | ? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | galactosylhydroxylysine bound in purified alpha1-chain of chicken skin collagen | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | product is glucosylgalactosylhydroxylysine-collagen | ? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | enzyme recognizes probably not only the carbohydrate side chains but also the primary structure of collagen | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | product is glucosylgalactosylhydroxylysine-collagen | ? | |
UDP-glucose + D-galactosylhydroxylysine | in vitro | Homo sapiens | UDP + 1,2-D-glucosyl-D-galactosylhydroxylysine | - |
? | |
UDP-glucose + D-galactosylhydroxylysine | galactosylhydroxylysine purified from marine sponge collagen | Homo sapiens | UDP + 1,2-D-glucosyl-D-galactosylhydroxylysine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
10% loss of activity within 18 h, soluble enzyme, after DEAE-ion exchange chromatography | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | soluble enzyme | Homo sapiens |
6.5 | 8 | - |
Homo sapiens |
7 | 8 | membrane-bound enzyme | Homo sapiens |