Cloned (Comment) | Organism |
---|---|
full-length LH3 and the N-terminal 30 kDa fragment A with GGT activity is cloned, sequenced and expressed in High Five insect cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km: 4 mg/ml, denatured citrate soluble rat skin collagen | Homo sapiens | |
0.15 | - |
UDP-glucose | recombinant LH3/GGT | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
LH3 with GGT activity, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | there must be an additional glucosyltransferase to LH3 that is responsible for most of the collagen glucosylation in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | structural domain fragment A of LH3 with GGT activity contains 2 N-glycosylation sites | Homo sapiens |
Purification (Comment) | Organism |
---|---|
His-tagged recombinant LH3/GGT/GT and N-terminal 30kDa fragment A of LH3 with GGT activity expressed in High Five insect cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | multifunctional lysyl hydroxylase isoform 3, LH3 protein, possesses lysyl hydroxylase, collagen glucosyltransferase and galactosyltransferase activities | Homo sapiens | ? | - |
? | |
additional information | GGT and GT activities reside in the N-terminal 30.4 kDa structural domain fragment A of LH3, which plays no role in hydroxylase activity, only trace amounts of GT activity of LH3 | Homo sapiens | ? | - |
? | |
additional information | there must be an additional glucosyltransferase to LH3 that is responsible for most of the collagen glucosylation in vivo | Homo sapiens | ? | - |
? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | heat-denatured citrate-soluble rat skin collagen | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 80000-85000, recombinant LH3 with GGT/GT activities, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | GGT activity is associated with lysyl hydroxylase isoform 3, LH3, EC 1.14.11.4 | Homo sapiens |