Literature summary for 2.4.1.41 extracted from

DeCicco RePass, M.A.; Bhat, N.; Heimburg-Molinaro, J.; Bunnell, S.; Cummings, R.D.; Ward, H.D.
Molecular cloning, expression, and characterization of UDP N-acetyl-alpha-D-galactosamine Polypeptide N-acetylgalactosaminyltransferase 4 from Cryptosporidium parvum (2018), Mol. Biochem. Parasitol., 221, 56-65 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in HCT-8 cell and HEK-293 cell	Cryptosporidium parvum

Crystallization (Commentary)

Crystallization (Comment)	Organism
homology modeling of structure	Cryptosporidium parvum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.052	-	UDP-N-acetyl-alpha-D-galactosamine	pH 7.5, 23°C	Cryptosporidium parvum

Organism

Organism	UniProt	Comment	Textmining
Cryptosporidium parvum	Q6TBQ8	isoform GalNAcT4	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Cryptosporidium parvum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	enzyme exhibits a strong preference for UDP-GalNAc over other nucleotide sugar donors, with no activity against UDP-Gal, and 40% activity of that seen toward UDP-GalNAc toward UDP-GlcNAc	Cryptosporidium parvum	?	-	-
UDP-N-acetyl-alpha-D-galactosamine + EA2 peptide	EA peptide, i.e. PTTDSTTPAPTTK	Cryptosporidium parvum	UDP + N-acetyl-alpha-D-galactosaminyl-EA2 peptide	-	?
UDP-N-acetyl-alpha-D-galactosamine + gp40-derived peptide	-	Cryptosporidium parvum	UDP + N-acetyl-alpha-D-galactosaminyl-gp40-derived peptide	-	?

Subunits

Subunits	Comment	Organism
?	x * 103000, SDS-PAGE of recombinant FLAG-tagged and glycosylated protein	Cryptosporidium parvum

Synonyms

Synonyms	Comment	Organism
GalNAcT4	-	Cryptosporidium parvum

Expression

Organism	Comment	Expression
Cryptosporidium parvum	GalNAc-T4 is differentially expressed during Cryptosporidium parvum infection of HCT-8 cells in vitro. Maximal expression is observed at 6 and 24 h post-infection with no expression seen at 48 and 72 h post-infection	additional information

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Release 2023.1 (January 2023)