Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
the pimA gene is cloned into the pET82a vector and the recombinant plasmid is transformed into Escherichia coli BL21 (DE3) | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
GDP-alpha-D-mannose | pH 7.0, 37° | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal concentration is 2-3 mM | Mycobacterium tuberculosis | |
Mg2+ | about 90% increase of activtiy in presence of 2-3 mM Mg2+ | Mycobacterium tuberculosis | |
Mn2+ | may partly substitute for Mg2+ | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
- |
Mycobacterium tuberculosis |
42000 | - |
x * 42000, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol | Mycobacterium tuberculosis | - |
GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol | Mycobacterium tuberculosis H37Rv | - |
GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
GDP-mannose + phosphatidyl-myo-inositol | Mycobacterium tuberculosis | PimA plays an essential role in the growth of mycobacteria | GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol | - |
? | |
GDP-mannose + phosphatidyl-myo-inositol | Mycobacterium tuberculosis H37Rv | PimA plays an essential role in the growth of mycobacteria | GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
H37Rv | - |
Mycobacterium tuberculosis | P9WMZ5 | - |
- |
Mycobacterium tuberculosis H37Rv | - |
H37Rv | - |
Mycobacterium tuberculosis H37Rv | P9WMZ5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mycobacterium tuberculosis |
9.4 | - |
pH 7.0, 37°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol | - |
Mycobacterium tuberculosis | GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol | - |
Mycobacterium tuberculosis H37Rv | GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
GDP-alpha-D-mannose + 1-phosphatidyl-myo-inositol | substrate bovine phosphatidylinositol | Mycobacterium tuberculosis | GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
GDP-alpha-D-mannose + 1-phosphatidyl-myo-inositol | substrate bovine phosphatidylinositol | Mycobacterium tuberculosis H37Rv | GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
GDP-mannose + phosphatidyl-myo-inositol | - |
Mycobacterium tuberculosis | GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol | - |
? | |
GDP-mannose + phosphatidyl-myo-inositol | PimA plays an essential role in the growth of mycobacteria | Mycobacterium tuberculosis | GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol | - |
? | |
GDP-mannose + phosphatidyl-myo-inositol | - |
Mycobacterium tuberculosis H37Rv | GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol | - |
? | |
GDP-mannose + phosphatidyl-myo-inositol | PimA plays an essential role in the growth of mycobacteria | Mycobacterium tuberculosis H37Rv | GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Mycobacterium tuberculosis |
? | x * 42605, MALDI-TOF, x * 42739, calculated | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
GDP-mannosyltransferase | - |
Mycobacterium tuberculosis |
PimA | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Mycobacterium tuberculosis |