Literature summary for 2.4.1.227 extracted from

Mitachi, K.; Yun, H.; Gillman, C.; Skorupinska-Tudek, K.; Swiezewska, E.; Clemons, W.; Kurosu, M.
Substrate tolerance of bacterial glycosyltransferase MurG novel fluorescence-based assays (2019), ACS Infect. Dis., 6, 1501-1516 .

Search for more literature for 2.4.1.227

Cloned(Commentary)

Cloned (Comment)	Organism
the murG gene is cloned with an N-terminal His6 tag into a pET33b vector. The plasmid is transformed and expressed in Escherichia coli NiCo21(DE3) competent cells	Mycolicibacterium smegmatis
the murG gene is cloned with an N-terminal His6 tag into a pET33b vector. The plasmid was transformed and expressed in Escherichia coli NiCo21(DE3) competent cells	Hydrogenivirga sp. 128-5-R1-1

Inhibitors

Inhibitors	Comment	Organism	Structure
nikkomycin Z	-	Hydrogenivirga sp. 128-5-R1-1
nikkomycin Z	-	Mycolicibacterium smegmatis
Polyoxin D	-	Hydrogenivirga sp. 128-5-R1-1
Polyoxin D	-	Mycolicibacterium smegmatis
PyDT-1	-	Mycolicibacterium smegmatis
ramoplanin A2	-	Hydrogenivirga sp. 128-5-R1-1
ramoplanin A2	-	Mycolicibacterium smegmatis
ristocetin A	strong antimycobacterial MurG inhibitor	Hydrogenivirga sp. 128-5-R1-1
ristocetin A	strong antimycobacterial MurG inhibitor	Mycolicibacterium smegmatis
ToXa-1	-	Mycolicibacterium smegmatis

Organism

Organism	UniProt	Comment	Textmining
Hydrogenivirga sp. 128-5-R1-1	A8US35	-	-
Mycolicibacterium smegmatis	-	-	-
Mycolicibacterium smegmatis ATCC 607	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mycolicibacterium smegmatis
-	Hydrogenivirga sp. 128-5-R1-1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	fluorescence-based assay	Mycolicibacterium smegmatis
additional information	-	fluorescence-based assay	Hydrogenivirga sp. 128-5-R1-1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-alpha-D-glucosamine + C55-lipid I-Nepsilon-C6-dansyl thiourea	-	Mycolicibacterium smegmatis	UDP + C55-lipid II-Nepsilon-C6-dansyl thiourea	-	?
UDP-N-acetyl-alpha-D-glucosamine + C55-lipid I-Nepsilon-C6-dansyl thiourea	-	Hydrogenivirga sp. 128-5-R1-1	UDP + C55-lipid II-Nepsilon-C6-dansyl thiourea	-	?
UDP-N-acetyl-alpha-D-glucosamine + C55-lipid I-Nepsilon-C6-dansyl thiourea	-	Mycolicibacterium smegmatis ATCC 607	UDP + C55-lipid II-Nepsilon-C6-dansyl thiourea	-	?

Synonyms

Synonyms	Comment	Organism
HyMurG	-	Hydrogenivirga sp. 128-5-R1-1
MsmegMurG	-	Mycolicibacterium smegmatis
MurG	-	Mycolicibacterium smegmatis
MurG	-	Hydrogenivirga sp. 128-5-R1-1

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00096	-	pH 8.0, 37°C	Hydrogenivirga sp. 128-5-R1-1	ristocetin A
0.0014	-	pH 8.0, 37°C	Mycolicibacterium smegmatis	ristocetin A
0.002	-	pH 8.0, 37°C	Mycolicibacterium smegmatis	ToXa-1
0.0035	-	pH 8.0, 37°C	Mycolicibacterium smegmatis	PyDT-1
0.0087	-	pH 8.0, 37°C	Hydrogenivirga sp. 128-5-R1-1	nikkomycin Z
0.0095	-	pH 8.0, 37°C	Mycolicibacterium smegmatis	nikkomycin Z
0.0224	-	pH 8.0, 37°C	Hydrogenivirga sp. 128-5-R1-1	ramoplanin A2
0.0235	-	pH 8.0, 37°C	Mycolicibacterium smegmatis	ramoplanin A2
0.0508	-	pH 8.0, 37°C	Hydrogenivirga sp. 128-5-R1-1	Polyoxin D
0.055	-	pH 8.0, 37°C	Mycolicibacterium smegmatis	Polyoxin D

General Information

General Information	Comment	Organism
metabolism	the enzyme is an essential bacterial glycosyltransferase that catalyses the GlcNAc-transformation of lipid I to lipid II during peptidoglycan biosynthesis	Mycolicibacterium smegmatis
metabolism	the enzyme is an essential bacterial glycosyltransferase that catalyses the GlcNAc-transformation of lipid I to lipid II during peptidoglycan biosynthesis	Hydrogenivirga sp. 128-5-R1-1

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Release 2023.1 (January 2023)