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Literature summary for 2.4.1.18 extracted from
- Comparison of the structural properties and nutritional fraction of corn starch treated with thermophilic GH13 and GH57 alpha-glucan branching enzymes (2019), Foods, 8, 452 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pyrococcus horikoshii |
| expressed in Escherichia coli BL21(DE3) cells | Caldicellulosiruptor bescii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor bescii | B9MPC3 | - |
- |
| Caldicellulosiruptor bescii ATCC BAA-1888 | B9MPC3 | - |
- |
| Pyrococcus horikoshii | O50094 | - |
- |
| Pyrococcus horikoshii OT-3 | O50094 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Pyrococcus horikoshii |
| Ni-NTA agarose column chromatography | Caldicellulosiruptor bescii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin | - |
Pyrococcus horikoshii | amylopectin containing alpha-1,6-glucosidic linkages | - |
? |  |
| amylopectin | - |
Caldicellulosiruptor bescii | amylopectin containing alpha-1,6-glucosidic linkages | - |
? | |
| amylopectin | - |
Caldicellulosiruptor bescii ATCC BAA-1888 | amylopectin containing alpha-1,6-glucosidic linkages | - |
? | |
| amylopectin | - |
Pyrococcus horikoshii OT-3 | amylopectin containing alpha-1,6-glucosidic linkages | - |
? | |
| amylose | - |
Caldicellulosiruptor bescii | amylose containing alpha-1,6-glucosidic linkages | - |
? | |
| amylose | the enzyme shows limited activity for substrates containing only alpha-1,4 linkages such as amylose | Pyrococcus horikoshii | amylose containing alpha-1,6-glucosidic linkages | - |
? | |
| amylose | - |
Caldicellulosiruptor bescii ATCC BAA-1888 | amylose containing alpha-1,6-glucosidic linkages | - |
? | |
| amylose | the enzyme shows limited activity for substrates containing only alpha-1,4 linkages such as amylose | Pyrococcus horikoshii OT-3 | amylose containing alpha-1,6-glucosidic linkages | - |
? | |
| corn starch | - |
Pyrococcus horikoshii | corn starch containing alpha-1,6-glucosidic linkages | - |
? | |
| corn starch | - |
Caldicellulosiruptor bescii | corn starch containing alpha-1,6-glucosidic linkages | - |
? | |
| corn starch | - |
Caldicellulosiruptor bescii ATCC BAA-1888 | corn starch containing alpha-1,6-glucosidic linkages | - |
? | |
| corn starch | - |
Pyrococcus horikoshii OT-3 | corn starch containing alpha-1,6-glucosidic linkages | - |
? | |
| high-amylose corn starch | - |
Pyrococcus horikoshii | high-amylose corn starch containing alpha-1,6-glucosidic linkages | - |
? | |
| high-amylose corn starch | - |
Caldicellulosiruptor bescii | high-amylose corn starch containing alpha-1,6-glucosidic linkages | - |
? | |
| high-amylose corn starch | - |
Caldicellulosiruptor bescii ATCC BAA-1888 | high-amylose corn starch containing alpha-1,6-glucosidic linkages | - |
? | |
| high-amylose corn starch | - |
Pyrococcus horikoshii OT-3 | high-amylose corn starch containing alpha-1,6-glucosidic linkages | - |
? | |
| additional information | the branching activity of the enzyme contains both alpha-1,4 and alpha-1,6 linkages | Caldicellulosiruptor bescii | ? | - |
- |
|
| additional information | the branching activity of the enzyme contains both alpha-1,4 and alpha-1,6 linkages | Caldicellulosiruptor bescii ATCC BAA-1888 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE | Pyrococcus horikoshii |
| ? | x * 70000, SDS-PAGE | Caldicellulosiruptor bescii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| amylo-(1,4->1,6)-transglycosylase | - |
Pyrococcus horikoshii |
| amylo-(1,4->1,6)-transglycosylase | - |
Caldicellulosiruptor bescii |
| GBE | - |
Pyrococcus horikoshii |
| GBE | - |
Caldicellulosiruptor bescii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Pyrococcus horikoshii |
| 60 | - |
- |
Caldicellulosiruptor bescii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pyrococcus horikoshii |
| 6 | - |
- |
Caldicellulosiruptor bescii |
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Release 2023.1 (January 2023)
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