Literature summary for 2.4.1.18 extracted from

Ban, X.; Wu, J.; Kaustubh, B.; Lahiri, P.; Dhoble, A.S.; Gu, Z.; Li, C.; Cheng, L.; Hong, Y.; Tong, Y.; Li, Z.
Additional salt bridges improve the thermostability of 1,4-alpha-glucan branching enzyme (2020), Food Chem., 316, 126348 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Parageobacillus thermoglucosidasius

Protein Variants

Protein Variants	Comment	Organism
I571D	the mutant shows increased thermostability and activity nearly identical to that of the wild type enzyme	Parageobacillus thermoglucosidasius
Q231K	the mutant shows increased thermostability and activity nearly identical to that of the wild type enzyme	Parageobacillus thermoglucosidasius
Q231R	the mutant shows increased thermostability and activity nearly identical to that of the wild type enzyme	Parageobacillus thermoglucosidasius
T339D	the mutant shows increased thermostability and activity nearly identical to that of the wild type enzyme	Parageobacillus thermoglucosidasius
T339E	the mutant shows increased thermostability and activity nearly identical to that of the wild type enzyme	Parageobacillus thermoglucosidasius

Organism

Organism	UniProt	Comment	Textmining
Parageobacillus thermoglucosidasius	A0A068F9H3	-	-
Parageobacillus thermoglucosidasius STB02	A0A068F9H3	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography	Parageobacillus thermoglucosidasius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.281	-	mutant enzyme I571D, pH and temperature not specified in the publication	Parageobacillus thermoglucosidasius
0.282	-	wild type enzyme, pH and temperature not specified in the publication	Parageobacillus thermoglucosidasius
0.293	-	mutant enzyme T339E, pH and temperature not specified in the publication	Parageobacillus thermoglucosidasius
0.295	-	mutant enzyme Q231R, pH and temperature not specified in the publication	Parageobacillus thermoglucosidasius
0.296	-	mutant enzyme T339D, pH and temperature not specified in the publication	Parageobacillus thermoglucosidasius
0.298	-	mutant enzyme Q231K, pH and temperature not specified in the publication	Parageobacillus thermoglucosidasius

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
amylopectin	-	Parageobacillus thermoglucosidasius	amylopectin containing alpha-1,6-glucosidic linkages	-	?
amylopectin	-	Parageobacillus thermoglucosidasius STB02	amylopectin containing alpha-1,6-glucosidic linkages	-	?
amylose	-	Parageobacillus thermoglucosidasius	amylose containing alpha-1,6-glucosidic linkages	-	?
amylose	-	Parageobacillus thermoglucosidasius STB02	amylose containing alpha-1,6-glucosidic linkages	-	?

Synonyms

Synonyms	Comment	Organism
GBE	-	Parageobacillus thermoglucosidasius

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	the wild type enzyme shows a half-life of 42.1 min at 60°C and retains less than 20% activity after 2 h at 60°C. Mutant enzyme Q231R shows a half-life of 53.2 min at 60°C and retains about 31% activity after 2 h at 60°C. Mutant enzyme Q231K shows a half-life of 53.6 min at 60°C and retains about 33% activity after 2 h at 60°C. Mutant enzyme T339E shows a half-life of 49.1 min at 60°C and retains about 28% activity after 2 h at 60°C. Mutant enzyme T339D shows a half-life of 50.4 min at 60°C and retains about 23% activity after 2 h at 60°C. Mutant enzyme I571D shows a half-life of 64.2 min at 60°C and retains about 36% activity after 2 h at 60°C	Parageobacillus thermoglucosidasius

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Release 2023.1 (January 2023)