Cloned (Comment) | Organism |
---|---|
cotransfection and expression of Epstein-Barr virus (EBV) miRNA miR-BART1-5p and GCNT3 in HEK-293T and SGC7901 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockdown of GCNT3 using GCNT3-specific small interfering RNAs: siGCNT3-1 (GCUACUGCGAGCUGUGUAUTT), and siGCNT3-2 (GCUCAGUGCCGUGGAAAUATT). Reduction of the expression of endogenous GCNT3 by siRNA transfection in SGC7901 and BGC823 cells. Epstein-Barr virus (EBV) miRNA miR-BART1-5p specifically targets GCNT3. miR-BART1-5p suppresses GCNT3 expression, cell proliferation, and migration in EBVnGC, and the MiR-BART1-5p inhibitor increases GCNT3 expression, cell proliferation, and migration in transfected GT39 and GT38 cells. NF-kappaB can activate the expression of multiple miR-BARTs, including miR-BART1-5p | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O95395 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
AGS cell | an EBVnGC cell line | Homo sapiens | - |
BGC-823 cell | an EBVnGC cell line | Homo sapiens | - |
gastric cancer cell | GCNT3 expression in Epstein-Barr virus (EBV)-associated gastric cancer cells and tissues is lower than in EBV-negative gastric cancer cells (EBVnGC) and tissues, and high expression is significantly associated with advanced tumor-lymph node metastasis. GCNT3 is closely related to the ERK signaling pathway and epithelial mesenchymal transition (EMT), regulating cell proliferation, migration, and invasion | Homo sapiens | - |
GT-38 cell | an EBVaGC cell line | Homo sapiens | - |
GT-39 cell | an EBVaGC cell line | Homo sapiens | - |
HGC-27 cell | an EBVnGC cell line | Homo sapiens | - |
additional information | GCNT3 is highly expressed in both NSCLC tissues and cell lines, and higher expression is significantly associated with advanced tumor lymph node metastasis (TNM) stage, positive lymph node metastasis, and poor overall survival. GCNT3 expression is associated with lymph node metastasis and age. But the expression level of GCNT3 is not correlated with gender, tumor location, differentiation grade, etc. Expression of GCNT3 is lower in EBVaGC cells and tissues than that in EBVnGC cells and tissues | Homo sapiens | - |
non-small cell lung cancer cell | GCNT3 is highly expressed in both NSCLC tissues and cell lines | Homo sapiens | - |
SGC-7901 cell | an EBVnGC cell line | Homo sapiens | - |
SNU-719 cell | an EBVaGC cell line | Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
2beta-1,6-acetylglucosaminyltransferase | - |
Homo sapiens |
C2/4GnT | - |
Homo sapiens |
C24GNT | - |
Homo sapiens |
C2GnT2 | - |
Homo sapiens |
C2GNTM | - |
Homo sapiens |
core 2beta-1,6-acetylglucosaminyltransferase | - |
Homo sapiens |
gcnt3 | - |
Homo sapiens |
GNTM | - |
Homo sapiens |
More | see also EC 2.4.1.102 | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | miR-BART1-5p directly targets GCNT3. In addition, miR-BART1-5p mimics transfection is observed to reduce cell proliferation and migration, while miR-BART1-5p inhibitor increases cell proliferation and migration following transfection. In conclusion, both miR-BART1-5p and knockdown of GCNT3 inhibit cell proliferation and migration | Homo sapiens |
physiological function | O-glycan synthase glucosamine (N-acetyl)transferase 3 (GCNT3) is a mucin-type responsible for catalyzing core 2 and core 4 O-glycans and forming O-linked glycosylation in protein biosynthesis. Abnormal expression of GCNT3 promotes the progression of several human cancers. GCNT3 expression in Epstein-Barr virus (EBV)-associated gastric cancer cells and tissues is lower than in EBV-negative gastric cancer cells and tissues, and high expression is significantly associated with advanced tumor-lymph node metastasis. EBV may regulate GCNT3 by affecting the NF-kappaB signaling pathway. Patients with EBV-associated gastric cancer (EBVaGC) have a good survival rate. EBV potentially regulates GCNT3 by affecting the NF-kappaB signaling pathway | Homo sapiens |