Cloned (Comment) | Organism |
---|---|
optimized expression in Escherichia coli | Vitis vinifera |
General Stability | Organism |
---|---|
at least three freeze-thaw cycles can be tolerated without apparent loss of activity | Vitis vinifera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 0.01 mM, over 80% reduction in quercetin glucosylation activity | Vitis vinifera | |
Mn2+ | - |
Vitis vinifera | |
Zn2+ | - |
Vitis vinifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
quercetin | - |
Vitis vinifera | |
0.016 | - |
delphinidin | - |
Vitis vinifera | |
0.03 | - |
cyanidin | - |
Vitis vinifera | |
0.0357 | - |
malvidin | - |
Vitis vinifera | |
1.9 | - |
UDP-glucose | - |
Vitis vinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Vitis vinifera | the principal, if not only, role of UDP-glucose:flavonoid 3-O-glucosyltransferase is to glucosylate anthocyanidins in red fruit ripening | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | P51094 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Vitis vinifera |
Storage Stability | Organism |
---|---|
-20°C, recombinant enzyme is stable for at least 120 h | Vitis vinifera |
-80°C, recombinant enzyme is stable for many months | Vitis vinifera |
4°C, recombinant enzyme is stable for at least 120 h | Vitis vinifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the principal, if not only, role of UDP-glucose:flavonoid 3-O-glucosyltransferase is to glucosylate anthocyanidins in red fruit ripening | Vitis vinifera | ? | - |
? | |
additional information | the enzyme is responsible for a late step in anthocyanin biosynthesis | Vitis vinifera | ? | - |
? | |
UDP-alpha-D-glucose + cyanidin | - |
Vitis vinifera | UDP + cyanidin 3-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + quercetin | no glucosylation with UDP-galactose, UDP-xylose, UDP-glucuronic acid, UDP-mannose, ADP-glucose, CDP-glucose, GDP-glucose or TDP-glucose | Vitis vinifera | UDP + quercetin 3-O-beta-D-glucoside | - |
? | |
UDP-glucose + delphinidin | - |
Vitis vinifera | UDP + delphinidin 3-O-beta-D-glucoside | - |
? | |
UDP-glucose + delphinidin | - |
Vitis vinifera | UDP + delphinidin-3-O-beta-D-glucoside | - |
? | |
UDP-glucose + malvidin | - |
Vitis vinifera | UDP + malvidin 3-O-beta-D-glucoside | - |
? | |
UDP-glucose + malvidin | - |
Vitis vinifera | UDP + malvidin-3-O-beta-D-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose:flavonoid 3-O-glucosyltransferase | - |
Vitis vinifera |
UFGT | - |
Vitis vinifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Vitis vinifera |