Literature summary for 2.3.3.5 extracted from

Yan, Y.; Wang, H.; Zhu, S.; Wang, J.; Liu, X.; Lin, F.; Lu, J.
The methylcitrate cycle is required for development and virulence in the rice blast fungus Pyricularia oryzae (2019), Mol. Plant Microbe Interact., 32, 1148-1161 .

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Organism

Organism	UniProt	Comment	Textmining
Pyricularia oryzae	-	i.e. Pyricularia oryzae	-

Synonyms

Synonyms	Comment	Organism
Mcs1	-	Pyricularia oryzae

Expression

Organism	Comment	Expression
Pyricularia oryzae	the enzyme is up-regulated during appressorium formation and when grown on propionyl-CoA-producing carbon sources	up

General Information

General Information	Comment	Organism
malfunction	deletion of MCS1 (2-methylcitrate synthase) and MCL1 (2-methylisocitrate lyase) inhibits fungal growth on media containing both glucose and propionate, and media using propionate or propionyl-CoA-producing amino acids (valine, isoleucine, methionine, and threonine) as the sole carbon or nitrogen sources. The DELTAmcs1 mutant forms sparse aerial hyphae and does not produce conidia on complete medium, while the DELTAmcl1 mutant shows decreased conidiation. The aerial mycelium of DELTAmcs1 displays lowered NAD+/NADH ratio, reduced nitric oxide content, and down-regulated transcription of hydrophobin genes. DELTAmcl1 shows reduced appressorium turgor, severely delayed plant penetration, and weakened virulence. Addition of acetate recovers the growth of the wild type and DELTAmcs1 on medium containing both glucose and propionate and recovers the conidiation of both DELTAmcs1 and DELTAmcl1 on complete medium by reducing propionyl-CoA formation. Deletion of MCL1 together with ICL1, an isocitrate lyase gene in the glyoxylate cycle, greatly reduces the virulence of the mutant as compared to the single gene deletion mutants (DELTAicl1 and DELTAmcl1)	Pyricularia oryzae
metabolism	MCS1 is required for growth and conidiation in Magnaporthe oryzae. Mcs1 is required for propionate utilization	Pyricularia oryzae

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Release 2023.1 (January 2023)