Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leptospira interrogans |
gene cimA, DNA and amino acid sequence determination and analysis, expression as His6-tagged enzyme in Escherichia coli | Leptospira interrogans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isoleucine | - |
Leptospira interrogans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
pyruvate | recombinant enzyme, pH 8.0, 37°C | Leptospira interrogans | |
0.043 | - |
pyruvate | - |
Leptospira interrogans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, recombinant His6-tagged enzyme, SDS-PAGE | Leptospira interrogans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + pyruvate + H2O | Leptospira interrogans | the enzyme is involved in the threonine-independent biosynthesis of isoleucine via an alternative beta-methyl-D-malate pathway, the expression of cimA is transcriptionally regulated by isoleucine | CoA + (R)-citramalate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptospira interrogans | - |
serotype lai strain 56601 | - |
Leptospira interrogans | - |
serotype lai strain 56601, gene cimA or LA2350 | - |
Purification (Comment) | Organism |
---|---|
- |
Leptospira interrogans |
recombinant His6-tagged enzyme 14.3fold from Escherichia coli by nickel affinity chromatography to homogeneity | Leptospira interrogans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.174 | - |
crude extract of Escherichia coli recombinantly expressing CimA | Leptospira interrogans |
2.5 | - |
- |
Leptospira interrogans |
2.53 | - |
purified recombinant His6-tagged enzyme | Leptospira interrogans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + pyruvate | the enzyme is strictly specific for pyruvate as keto acid substrate | Leptospira interrogans | (3S)-citramalyl-CoA | - |
? | |
acetyl-CoA + pyruvate + H2O | the enzyme is involved in the threonine-independent biosynthesis of isoleucine via an alternative beta-methyl-D-malate pathway, the expression of cimA is transcriptionally regulated by isoleucine | Leptospira interrogans | CoA + (R)-citramalate | - |
? | |
acetyl-CoA + pyruvate + H2O | the enzyme is strictly specific for pyruvate as the keto acid substrate | Leptospira interrogans | CoA + (R)-citramalate | - |
? | |
additional information | LA2350 is a citramalate synthase without detectable alpha-isopropylmalate synthase activity | Leptospira interrogans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant His6-tagged enzyme, SDS-PAGE | Leptospira interrogans |
Synonyms | Comment | Organism |
---|---|---|
CimA | - |
Leptospira interrogans |
citramalate synthase | - |
Leptospira interrogans |
LA2350 | - |
Leptospira interrogans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Leptospira interrogans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature profile, overview | Leptospira interrogans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
40 | 80% of maximal activity within this range | Leptospira interrogans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.41 | - |
pyruvate | - |
Leptospira interrogans | |
2.41 | - |
pyruvate | recombinant enzyme, pH 8.0, 37°C | Leptospira interrogans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Leptospira interrogans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Leptospira interrogans |