Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) RIL star cells | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA | in the presence of Mg2+, or Mn2+, product inhibition by CoA is competitive versus acetyl-CoA but uncompetitive versus 2-oxoglutarate | Thermus thermophilus | |
EDTA | - |
Thermus thermophilus | |
L-lysine | competitive inhibitor versus 2-oxoglutarate up to a concentration of 1 mM | Thermus thermophilus | |
additional information | not inhibited by oxalate, malonyl-CoA, pantothenate | Thermus thermophilus | |
oxalglycine | competitive inhibition versus 2-oxoglutarate | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Thermus thermophilus | |
Mn2+ | activates | Thermus thermophilus | |
additional information | not activated by 50 mM Zn2+, 5 mM Ni2+ and 5 mM Co2+ | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + 2-oxoglutarate | - |
Thermus thermophilus | 2-hydroxybutane-1,2,4-tricarboxylate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetate | oxaloacetate is a slow alternative substrate | Thermus thermophilus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HCS | - |
Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermus thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
CoA | competitive inhibition mode, in 50 mM HEPES, pH 7.5, at 25°C | Thermus thermophilus | |
0.11 | - |
CoA | competitive inhibition mode, in 50 mM HEPES, pH 7.5, at 25°C | Thermus thermophilus | |
0.9 | - |
oxalglycine | in 50 mM HEPES, pH 7.5, at 25°C | Thermus thermophilus |