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Literature summary for 2.3.3.13 extracted from

  • Abe, T.; Toyokawa, Y.; Sugimoto, Y.; Azuma, H.; Tsukahara, K.; Nasuno, R.; Watanabe, D.; Tsukahara, M.; Takagi, H.
    Characterization of a new Saccharomyces cerevisiae isolated from Hibiscus flower and its mutant with L-leucine accumulation for awamori brewing (2019), Front. Genet., 10, 490 .
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
food industry in mutant strain T25 with L-leucine accumulation, a hetero allelic mutation in the LEU4 gene encoding the Gly516Ser variant alpha-isopropylmalate synthase is found. alpha-Isopropylmalate synthase activity of the Gly516Ser variant is less sensitive to feedback inhibition by L-leucine, leading to intracellular L-leucine accumulation. In a laboratory-scale test, awamori (a distilled alcoholic beverage made from steamed rice,) brewed with strain T25 shows higher concentrations of isoamyl alcohol and isoamyl acetate than that brewed with strain HC02-5-2. Such a combinatorial approach to yeast isolation, with whole genome analysis and metabolism-focused breeding, has the potentials to vary the quality of alcoholic beverages Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
G516S in mutant strain T25 with L-leucine accumulation, a hetero allelic mutation in the LEU4 gene encoding the Gly516Ser variant alpha-isopropylmalate synthase is found. alpha-Isopropylmalate synthase activity of the Gly516Ser variant is less sensitive to feedback inhibition by L-leucine, leading to intracellular L-leucine accumulation. In a laboratory-scale test, awamori (a distilled alcoholic beverage made from steamed rice,) brewed with strain T25 shows higher concentrations of isoamyl alcohol and isoamyl acetate than that brewed with strain HC02-5-2. Such a combinatorial approach to yeast isolation, with whole genome analysis and metabolism-focused breeding, has the potentials to vary the quality of alcoholic beverages Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae HC02-5-2
-
-
-

Synonyms

Synonyms Comment Organism
Alpha-isopropylmalate synthase
-
 Saccharomyces cerevisiae
IPMS
-
 Saccharomyces cerevisiae