Inhibitors | Comment | Organism | Structure |
---|---|---|---|
leucine | mechanism of feedback inhibition by Leu, binding of Leu to wild-type and feedback-resistant enzyme and its structural consequences | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
strain CV-19, and mutant strain CV-241 with a feed-back insensitive enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
leucine | pH 6.8, 0.4 mM acetyl-CoA, 0.1 mM 2-oxo-3-methylbutanoate, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.022 | - |
leucine | pH 6.8, 0.8 mM acetyl-CoA, 4 mM 2-oxo-3-methylbutanoate, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
3 | - |
leucine | pH 6.8, 0.4 mM acetyl-CoA, 0.1 mM 2-oxo-3-methylbutanoate, feedback-resistent enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
20 | - |
leucine | pH 6.8, 0.8 mM acetyl-CoA, 4 mM 2-oxo-3-methylbutanoate, feedback-resistent enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |