Application | Comment | Organism |
---|---|---|
medicine | deficiency of the mitochondrial isoform correlates with human metabolic disease, cytosolic isoform is potentially useful target for drugs aimed at lowering cholesterol levels | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3) | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | - |
Gallus gallus | |
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | - |
Homo sapiens | |
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00119 | - |
acetoacetyl-CoA | - |
Gallus gallus | |
0.0078 | - |
acetyl-CoA | mutant D159A | Gallus gallus | |
0.0081 | - |
acetyl-CoA | mutant D159A, acetyl-S-enzyme formation | Gallus gallus | |
0.014 | - |
acetyl-CoA | wild-type, acetyl-CoA hydrolysis | Gallus gallus | |
0.0227 | - |
acetyl-CoA | wild-type, acetyl-S-enzyme formation | Gallus gallus | |
0.033 | - |
acetyl-CoA | mutant D159A, acetyl-CoA hydrolysis | Gallus gallus | |
0.037 | - |
acetyl-CoA | mutant D203A | Gallus gallus | |
0.049 | - |
acetoacetyl-CoA | mutant D159A | Gallus gallus | |
0.066 | - |
acetoacetyl-CoA | mutant D99A | Gallus gallus | |
0.118 | - |
acetyl-CoA | mutant D64N | Gallus gallus | |
0.189 | - |
acetyl-CoA | mutant E63Q | Gallus gallus | |
0.207 | - |
acetyl-CoA | mutant D99A | Gallus gallus | |
0.29 | - |
acetyl-CoA | wild-type enzyme | Gallus gallus | |
0.319 | - |
acetyl-CoA | mutant D217A | Gallus gallus | |
0.354 | - |
acetyl-CoA | mutant E37Q | Gallus gallus | |
0.358 | - |
acetyl-CoA | mutant D282A | Gallus gallus | |
0.372 | - |
acetyl-CoA | mutant E121A | Gallus gallus | |
0.448 | - |
acetyl-CoA | mutant D203A, acetyl-S-enzyme formation | Gallus gallus | |
0.623 | - |
acetyl-CoA | mutant D99A, acetyl-S-enzyme formation | Gallus gallus | |
0.677 | - |
acetyl-CoA | mutant D124A | Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | cholesterogenic isoform | Gallus gallus | 5737 | - |
cytoplasm | cholesterogenic isoform | Homo sapiens | 5737 | - |
cytoplasm | cholesterogenic isoform | Saccharomyces cerevisiae | 5737 | - |
cytoplasm | cholesterogenic isoform | Methanothermobacter thermautotrophicus | 5737 | - |
cytoplasm | cholesterogenic isoform | Arabidopsis thaliana | 5737 | - |
cytoplasm | cholesterogenic isoform | Borreliella burgdorferi | 5737 | - |
cytoplasm | cholesterogenic isoform | Caenorhabditis elegans | 5737 | - |
cytoplasm | cholesterogenic isoform | Mus musculus | 5737 | - |
cytoplasm | cholesterogenic isoform | Pinus sylvestris | 5737 | - |
cytoplasm | cholesterogenic isoform | Rattus norvegicus | 5737 | - |
cytoplasm | cholesterogenic isoform | Schizosaccharomyces pombe | 5737 | - |
cytoplasm | cholesterogenic isoform | Sus scrofa | 5737 | - |
cytosol | - |
Mesocricetus auratus | 5829 | - |
cytosol | - |
Blattella germanica | 5829 | - |
cytosol | - |
Cricetulus griseus | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | cholesterogenic isoform | Gallus gallus | 5829 | - |
cytosol | cholesterogenic isoform | Homo sapiens | 5829 | - |
cytosol | cholesterogenic isoform | Saccharomyces cerevisiae | 5829 | - |
cytosol | cholesterogenic isoform | Methanothermobacter thermautotrophicus | 5829 | - |
cytosol | cholesterogenic isoform | Arabidopsis thaliana | 5829 | - |
cytosol | cholesterogenic isoform | Borreliella burgdorferi | 5829 | - |
cytosol | cholesterogenic isoform | Caenorhabditis elegans | 5829 | - |
cytosol | cholesterogenic isoform | Mus musculus | 5829 | - |
cytosol | cholesterogenic isoform | Pinus sylvestris | 5829 | - |
cytosol | cholesterogenic isoform | Rattus norvegicus | 5829 | - |
cytosol | cholesterogenic isoform | Schizosaccharomyces pombe | 5829 | - |
cytosol | cholesterogenic isoform | Sus scrofa | 5829 | - |
mitochondrion | ketogenic isoform | Gallus gallus | 5739 | - |
mitochondrion | ketogenic isoform | Homo sapiens | 5739 | - |
mitochondrion | ketogenic isoform | Saccharomyces cerevisiae | 5739 | - |
mitochondrion | ketogenic isoform | Methanothermobacter thermautotrophicus | 5739 | - |
mitochondrion | ketogenic isoform | Arabidopsis thaliana | 5739 | - |
mitochondrion | ketogenic isoform | Borreliella burgdorferi | 5739 | - |
mitochondrion | ketogenic isoform | Caenorhabditis elegans | 5739 | - |
mitochondrion | ketogenic isoform | Mus musculus | 5739 | - |
mitochondrion | ketogenic isoform | Pinus sylvestris | 5739 | - |
mitochondrion | ketogenic isoform | Rattus norvegicus | 5739 | - |
mitochondrion | ketogenic isoform | Schizosaccharomyces pombe | 5739 | - |
mitochondrion | ketogenic isoform | Sus scrofa | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | Methanothermobacter thermautotrophicus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Mesocricetus auratus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Arabidopsis thaliana | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Blattella germanica | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Borreliella burgdorferi | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Caenorhabditis elegans | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Cricetulus griseus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Gallus gallus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Homo sapiens | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Mus musculus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Pinus sylvestris | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Rattus norvegicus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Saccharomyces cerevisiae | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Schizosaccharomyces pombe | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Sus scrofa | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Gallus gallus | key intermediate in cholesterogenic and ketogenic pathways, cholesterogenic isoform in cytosol, ketogenic isoform in mitochondria, committed step for both pathways | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
acetyl-CoA + acetoacetyl-CoA + H2O | Homo sapiens | key intermediate in cholesterogenic and ketogenic pathways, cholesterogenic isoform in cytosol, ketogenic isoform in mitochondria, committed step for both pathways | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
acetyl-CoA + acetoacetyl-CoA + H2O | Saccharomyces cerevisiae | key intermediate in cholesterogenic and ketogenic pathways, cholesterogenic isoform in cytosol, ketogenic isoform in mitochondria, committed step for both pathways | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P54873 | - |
- |
Blattella germanica | P54870 | cockroach, cytosolic isoform 2 | - |
Blattella germanica | P54961 | cockroach, cytosolic isoform 1 | - |
Borreliella burgdorferi | O51626 | - |
- |
Caenorhabditis elegans | P54871 | - |
- |
Cricetulus griseus | P13704 | chinese hamster, cytosolic isoform | - |
Gallus gallus | - |
avian | - |
Gallus gallus | P23228 | chicken, cytosolic isoform | - |
Homo sapiens | - |
human | - |
Homo sapiens | P54868 | human, mitochondrial isoform | - |
Homo sapiens | Q01581 | human, cytosolic isoform | - |
Mesocricetus auratus | - |
syrian hamster, golden hamster | - |
Methanothermobacter thermautotrophicus | - |
- |
- |
Mus musculus | P54869 | mouse, mitochondrial isoform | - |
Pinus sylvestris | P93773 | pine | - |
Rattus norvegicus | P17425 | rat, cytosolic isoform | - |
Rattus norvegicus | P22791 | rat, mitochondrial isoform | - |
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae | P54839 | baker's yeast | - |
Schizosaccharomyces pombe | P54874 | - |
- |
Sus scrofa | O02734 | pig, mitochondrial isoform | - |
Purification (Comment) | Organism |
---|---|
several mutants partially purified | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Homo sapiens | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Methanothermobacter thermautotrophicus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Mesocricetus auratus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Arabidopsis thaliana | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Blattella germanica | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Borreliella burgdorferi | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Caenorhabditis elegans | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Cricetulus griseus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Homo sapiens | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Mus musculus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Pinus sylvestris | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Rattus norvegicus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Schizosaccharomyces pombe | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Sus scrofa | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | key intermediate in cholesterogenic and ketogenic pathways, cholesterogenic isoform in cytosol, ketogenic isoform in mitochondria, committed step for both pathways | Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
acetyl-CoA + acetoacetyl-CoA + H2O | key intermediate in cholesterogenic and ketogenic pathways, cholesterogenic isoform in cytosol, ketogenic isoform in mitochondria, committed step for both pathways | Homo sapiens | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
acetyl-CoA + acetoacetyl-CoA + H2O | key intermediate in cholesterogenic and ketogenic pathways, cholesterogenic isoform in cytosol, ketogenic isoform in mitochondria, committed step for both pathways | Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
ir | |
acetyl-CoA + H2O | - |
Gallus gallus | acetate + CoA | - |
? |