General Stability | Organism |
---|---|
enzyme is labile to freezing and thawing, stabilized by glycerol | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | residual activity 51% | Saccharomyces cerevisiae | |
arsenite | in presence of CoA, residual activity 25% | Saccharomyces cerevisiae | |
bromoacetyl-CoA | - |
Saccharomyces cerevisiae | |
CdSO4 | residual activity 9% | Saccharomyces cerevisiae | |
iodoacetamide | residual activity 84% | Saccharomyces cerevisiae | |
additional information | desulfo-CoA protects against bromoacetyl-CoA inhibition, but not against p-chloromercuribenzoate and N-ethylmaleimide | Saccharomyces cerevisiae | |
N-ethylmaleimide | residual activity 46% | Saccharomyces cerevisiae | |
NaAsO2 | residual activity 90% | Saccharomyces cerevisiae | |
p-chloromercuribenzoate | residual activity 16% | Saccharomyces cerevisiae | |
phenyl arsenious oxide | in presence of CoA, residual activity 41%; residual activity 75% | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | Saccharomyces cerevisiae | synthesis of isoprenes and steroids | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | synthesis of isoprenes and steroids | Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
additional information | acetyl-CoA analogues can act as substrates, acetoacyl-analogues are no substrates | Saccharomyces cerevisiae | ? | - |
? |