KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0409 | - |
beta-casein | - |
Homo sapiens | |
0.208 | - |
NQENVSPLTLLRLGN | 37°C, pH 7.4 | Homo sapiens | |
0.459 | - |
NQEQVSPLTLLKLGN | 37°C, pH 7.4 | Homo sapiens | |
0.782 | - |
LGPQSLVIG | 37°C, pH 7.4 | Homo sapiens | |
1.28 | - |
NQENVSPLTLLKLGN | 37°C, pH 7.4 | Homo sapiens | |
1.43 | - |
LGPQSKVIG | 37°C, pH 7.4 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinant factor XIIIa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-casein + glycine ethylester | - |
Homo sapiens | ? | - |
? | |
LGPQSKVIG + glycine-ethylester | i.e. K9, an optimized sequence based on beta-casein | Homo sapiens | ? | - |
? | |
LGPQSLVIG + glycine ethylester | i.e. K9(K7L), a modified optimized sequence based on beta-casein | Homo sapiens | ? | - |
? | |
additional information | study of enzyme specificity with Q-containing substrates based on beta-casein, K9-peptide and alpha2-antiplasmin. Factor XIIIa preferentially selects the Q2 residue for carrying out crosslinking processes.The E3 and Q4 provide supporting role in binding. When reaction occurs at Q2, then Q4 is sterically blocked. Deamidation of Q2 to E2 allows observation of reactivity at Q4. K12 position provides an additional favorable site of interaction with factor XIIIa surface | Homo sapiens | ? | - |
? | |
NNEQVSPLTLLKLGN + glycine ethylester | i.e. alpha2-antiplasmin peptide with modification Q2N | Homo sapiens | ? | - |
? | |
NQENVSPLTLLKLGN + glycine ethylester | i.e. alpha2-antiplasmin peptide with modification Q4N | Homo sapiens | ? | - |
? | |
NQENVSPLTLLLLGN + glycine ethylester | i.e. alpha2-antiplasmin peptide with modification Q4N, K12L | Homo sapiens | ? | - |
? | |
NQENVSPLTLLRLGN + glycine ethylester | i.e. alpha2-antiplasmin peptide with modification Q4N, K12R | Homo sapiens | ? | - |
? | |
NQEQVSPLTLLKLGN + glycine ethylester | i.e. alpha2-antiplasmin peptide | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
NQENVSPLTLLRLGN | 37°C, pH 7.4 | Homo sapiens | |
16.1 | - |
beta-casein | - |
Homo sapiens | |
44 | - |
LGPQSLVIG | 37°C, pH 7.4 | Homo sapiens | |
77.3 | - |
NQENVSPLTLLRLGN | 37°C, pH 7.4 | Homo sapiens | |
78 | - |
LGPQSKVIG | 37°C, pH 7.4 | Homo sapiens | |
147 | - |
NQENVSPLTLLKLGN | 37°C, pH 7.4 | Homo sapiens | |
175 | - |
NQEQVSPLTLLKLGN | 37°C, pH 7.4 | Homo sapiens |