Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Enterobacter cloacae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
gentamicin C1 | pH 7.6, 25°C | Enterobacter cloacae | |
0.003 | - |
gentamicin C2 | pH 7.6, 25°C | Enterobacter cloacae | |
0.015 | - |
sisomicin | pH 7.6, 25°C | Enterobacter cloacae | |
0.0802 | - |
kanamycin B | pH 7.6, 25°C | Enterobacter cloacae | |
0.106 | - |
tobramycin | pH 7.6, 25°C | Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + gentamicin C1 | - |
Enterobacter cloacae | CoA + N3'-acetylgentamicin C1 | - |
? | |
acetyl-CoA + gentamicin C2 | - |
Enterobacter cloacae | CoA + N3'-acetylgentamicin C2 | - |
? | |
acetyl-CoA + kanamycin B | - |
Enterobacter cloacae | CoA + N3'-acetylkanamycin B | - |
? | |
acetyl-CoA + sisomicin | - |
Enterobacter cloacae | CoA + N3'-acetyl-sisomicin | - |
? | |
acetyl-CoA + tobramycin | - |
Enterobacter cloacae | CoA + N3'-acetyltobramycin | - |
? | |
additional information | binding of aminoglycosides to AAC-VIa is enthalpically favored and entropically disfavored with a net result of favorable Gibbs energy. A net deprotonation of the enzyme, ligand, or both accompanies the formation of binary and ternary complexes. Poor substrates: kanamycn A, ribostamycin, neomycin B | Enterobacter cloacae | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
More | AAC-VIa displays a monomer-dimer equilibrium in the absence of substrates.The dimer formation is concentration-dependent between 5 microM and 100 microM enzyme with more dimeric form appearing at higher concentrations. Binding of ligands shifts the enzyme to monomeric state | Enterobacter cloacae |
Synonyms | Comment | Organism |
---|---|---|
AAC-VIa | - |
Enterobacter cloacae |
aminoglycoside N3-acetyltransferase-VIa | - |
Enterobacter cloacae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.7 | - |
gentamicin C1 | pH 7.6, 25°C | Enterobacter cloacae | |
14.5 | - |
kanamycin B | pH 7.6, 25°C | Enterobacter cloacae | |
18.4 | - |
gentamicin C2 | pH 7.6, 25°C | Enterobacter cloacae | |
24.5 | - |
sisomicin | pH 7.6, 25°C | Enterobacter cloacae | |
43.5 | - |
tobramycin | pH 7.6, 25°C | Enterobacter cloacae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
200 | - |
kanamycin B | pH 7.6, 25°C | Enterobacter cloacae | |
400 | - |
tobramycin | pH 7.6, 25°C | Enterobacter cloacae | |
1600 | - |
sisomicin | pH 7.6, 25°C | Enterobacter cloacae | |
4700 | - |
gentamicin C1 | pH 7.6, 25°C | Enterobacter cloacae | |
6100 | - |
gentamicin C2 | pH 7.6, 25°C | Enterobacter cloacae |