Cloned (Comment) | Organism |
---|---|
gene GCN5, quantitative RT-PCR enzyme expression analysis, recombinant ectopical expression of HA-tagged enzyme TgGCN5b | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
garcinol | isolated from Garcinia indica, inhibits GCN5-mediated lysine acetyltransferase activity and prevents the replication of the parasite. Treatment of tachyzoites with garcinol leads to a reduction of global lysine acetylation, particularly on histone H3 and TgGCN5b itself. transcriptome sequencing (RNA-seq), which reveals increasing aberrant gene expression coincident with increasing concentrations of garcinol. The majority of the genes that are most significantly affected by garcinol are also associated with TgGCN5b in a previously reported chromatin immunoprecipitation assay with microarray technology analysis. The dysregulated gene expression induced by garcinol significantly inhibits Toxoplasma tachyzoite replication, and the concentrations used exhibit no overt toxicity on human host cells. Garcinol also reduces autoacetylation of TgGCN5b, garcinol treatment results in a 65% reduction in the acetylation level of HA-tagged TgGCN5b. Garcinol-mediated changes in the parasite transcriptome, overview | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Toxoplasma gondii | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + [histone H3]-L-lysine9 | Toxoplasma gondii | - |
CoA + [histone H3]-N6-acetyl-L-lysine9 | - |
? | |
acetyl-CoA + [protein]-L-lysine | Toxoplasma gondii | - |
CoA + [protein]-N6-acetyl-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | Q9NIS0 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type and recombinant HA-tagged enzyme | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Toxoplasma gondii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + [histone H3]-L-lysine9 | - |
Toxoplasma gondii | CoA + [histone H3]-N6-acetyl-L-lysine9 | - |
? | |
acetyl-CoA + [protein]-L-lysine | - |
Toxoplasma gondii | CoA + [protein]-N6-acetyl-L-lysine | - |
? | |
additional information | TgGCN5b performs autoacetylation. Proteome-wide acetylome analyses of Toxoplasma tachyzoites identifies seven acetylated lysines on TgGCN5b, i.e. K811, K857, K941, K989, K997, K1002, and K1027 | Toxoplasma gondii | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
GCN5 family KAT | - |
Toxoplasma gondii |
GCN5 lysine acetyltransferase | - |
Toxoplasma gondii |
TgGCN5b | - |
Toxoplasma gondii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Toxoplasma gondii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Toxoplasma gondii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Toxoplasma gondii |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme TgGCN5b is the only nuclear GCN5 family KAT known to be required for Toxoplasma tachyzoite replication | Toxoplasma gondii |