Application | Comment | Organism |
---|---|---|
pharmacology | synthase III is a target for drug development against multi-drug resistant strains | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
synthase III, overexpression of His-tagged FabH in Escherichia coli BL21 (DE3) | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-phenyl-5-phenylimino-[1,2,4]dithiazolidin-3-one | i.e. HR19; strong, synthase III | Staphylococcus aureus | |
5-chloro-4-phenyl-[1,2]-dithiol-3-one | complete inhibition at 0.01 nM, synthase III; i.e. HR12 | Staphylococcus aureus | |
cerulenin | weak, synthase III | Staphylococcus aureus | |
thiolactomycin | synthase III; weak | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00032 | - |
isobutyryl-CoA | synthase III | Staphylococcus aureus | |
0.00232 | - |
butyryl-CoA | synthase III | Staphylococcus aureus | |
0.00618 | - |
acetyl-CoA | synthase III | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence alignment | Staphylococcus aureus |
37000 | - |
2 * 37000, recombinant synthase III, SDS-PAGE | Staphylococcus aureus |
67600 | - |
gel filtration | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Staphylococcus aureus | initiation of fatty acid biosynthesis | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
ir | |
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Staphylococcus aureus | part of fatty acid synthase II | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | P99159 | synthase III, gene fabH | - |
Staphylococcus aureus | P99159 | strain ATCC 35556 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged synthase III from E. coli | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | ? | - |
? | |
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | synthase III | Staphylococcus aureus | acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
ir | |
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | initiation of fatty acid biosynthesis | Staphylococcus aureus | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
ir | |
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | part of fatty acid synthase II | Staphylococcus aureus | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
ir | |
butyryl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | ? | - |
? | |
butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | synthase III | Staphylococcus aureus | beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
isobutyryl-CoA + malonyl-[acyl-carrier protein] | synthase III | Staphylococcus aureus | ? | - |
? | |
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, recombinant synthase III, SDS-PAGE | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
synthase III | Staphylococcus aureus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8 | synthase III | Staphylococcus aureus |