Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
General Stability | Organism |
---|---|
temperature inactivation readily reversible | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
malonyl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
0.044 | - |
propionyl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
0.051 | - |
succinyl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
0.068 | - |
isobutyryl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
0.083 | - |
beta-hydroxybutyryl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
0.13 | - |
acetyl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
0.14 | - |
CoA | 25°C, pH 7.5 | Thermotoga maritima | |
0.18 | - |
glutaryl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
0.24 | - |
L-homoserine | 25°C, pH 7.5 | Thermotoga maritima | |
1.7 | - |
O-acetylhomoserine | 25°C, pH 7.5 | Thermotoga maritima | |
280 | - |
D-homoserine | 25°C, pH 7.5 | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine | enzyme utilizes a ping-pong kinetic mechanism in which the acetate group of acetyl-CoA is initially transferred to an enzyme nucleophile before subsequent transfer to homoserine | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + D-homoserine | - |
Thermotoga maritima | CoA + O-acetyl-D-homoserine | - |
? | |
acetyl-CoA + L-homoserine | the enzyme utilizes a ping-pong kinetic mechanism in which the acetate group of acetyl-CoA is initially transferred to an enzyme nucleophile before subsequent transfer to homoserine | Thermotoga maritima | CoA + O-acetyl-L-homoserine | - |
r | |
beta-hydroxybutyryl-CoA + L-homoserine | - |
Thermotoga maritima | CoA + beta-hydroxybutyryl-L-homoserine | - |
? | |
glutaryl-CoA + L-homoserine | - |
Thermotoga maritima | CoA + O-glutaryl-L-homoserine | - |
? | |
isobutyryl-CoA + L-homoserine | - |
Thermotoga maritima | CoA + O-isobutyryl-L-homoserine | - |
? | |
malonyl-CoA + L-homoserine | - |
Thermotoga maritima | CoA + O-malonyl-L-homoserine | - |
? | |
propionyl-CoA + L-homoserine | - |
Thermotoga maritima | CoA + O-propionyl-L-homoserine | - |
? | |
succinyl-CoA + L-homoserine | - |
Thermotoga maritima | CoA + O-succinyl-L-homoserine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TmHTS | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | - |
Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
malonyl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
0.12 | - |
beta-hydroxybutyryl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
0.19 | - |
succinyl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
0.19 | - |
isobutyryl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
0.25 | - |
glutaryl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
1.9 | - |
CoA | 25°C, pH 7.5 | Thermotoga maritima | |
1.9 | - |
O-acetylhomoserine | 25°C, pH 7.5 | Thermotoga maritima | |
2.6 | - |
propionyl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
16 | - |
D-homoserine | 25°C, pH 7.5 | Thermotoga maritima | |
23 | - |
acetyl-CoA | 25°C, pH 7.5 | Thermotoga maritima | |
23 | - |
L-homoserine | 25°C, pH 7.5 | Thermotoga maritima |