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Literature summary for 2.3.1.297 extracted from
- Solubilization and partial characterization of fatty acyl-CoA sphingosine acyltransferase (ceramide synthetase) from rat liver and brain (1986), Biochim. Biophys. Acta, 877, 334-341 .
General Stability
| General Stability | Organism |
|---|---|
| high concentrations of glycerol are essential in preventing deactivation of the enzyme | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the enzyme kinetics are not exactly conform with Michaelis-Menten kinetics, the curve is hyperbolic or the activity of lignoceroyl-CoA : sphingosine lignoceroyltransferase in the liver preparation increases in a somewhat sigmoidal fashion with increasing lignoceroyl-CoA concentration up to about 0.004 mM | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingosine + lignoceroyl-CoA | Rattus norvegicus | - |
N-lignoceroylsphingosine + CoA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 2.7 and 4.9fold from liver and brain microsomes partially, respectively, by gel filtration | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several months | Rattus norvegicus |
| 4°C, 48 h, liver enzyme loses 70% of activity, brain enzyme looes 41% of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingosine + lignoceroyl-CoA | - |
Rattus norvegicus | N-lignoceroylsphingosine + CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ceramide synthetase | - |
Rattus norvegicus |
| lignoceroyl-CoA : sphingosine lignoceroyltransferase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Rattus norvegicus |
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