Cloned (Comment) | Organism |
---|---|
genes LAG1, LAC1 and LIP1, encodes the three subunits of the hetereotrimeric enzyme, sequence comparisons, recombinant expression of wild-type and point/insertion mutant FLAG- and/or c-Myc-tagged subunits in Saccharomyces cerevisiae strains, overview | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | topology study of Lag1p and Lac1p using insertion of glycosylation sites and fXa (factor Xa)-cleavage sites in the loops between the predicted transmembrane segments, hydropathy profiles of Lag1p and Lac1p and positions of fusion insertions, method, overview | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | transmembrane topology of topology of the Lag1p and Lac1p subunits in yeast using insertion of glycosylation sites and factor Xa-cleavage sites in the loops between the predicted transmembrane segments, method, overview. The N- and C-termini of the proteins are in the cytoplasm and eight putative membrane-spanning domains are identified in Lag1p and Lac1p. The conserved Lag motif, potentially containing the active site, is most likely embedded in the membrane | Saccharomyces cerevisiae | 5789 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P28496 AND P38703 AND Q03579 | encoding subunits Lac1p, Lag1p, and Lip1p | - |
Saccharomyces cerevisiae RH382 | P28496 AND P38703 AND Q03579 | encoding subunits Lac1p, Lag1p, and Lip1p | - |
Synonyms | Comment | Organism |
---|---|---|
ceramide synthase | - |
Saccharomyces cerevisiae |
Lac1p/Lag1p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
additional information | the conserved Lag motif, potentially containing the active site, is most likely embedded in the membrane. Histidine and aspartic acid residues in the Lag motif are essential for the function of Lag1p in vivo | Saccharomyces cerevisiae |
physiological function | proteins Lag1p, Lac1p, and Lip1p are required for ceramide synthase activity in Saccharomyces cerevisiae | Saccharomyces cerevisiae |