Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces griseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | EDTA (5 mM) has no effect on the reaction | Streptomyces griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2046 | - |
glycerone phosphate | pH 7.5, 15°C | Streptomyces griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metals are required for the activity | Streptomyces griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37700 | - |
SDS-PAGE | Streptomyces griseus |
45400 | - |
gel filtration | Streptomyces griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate | Streptomyces griseus | AfsA is the key enzyme for A factor biosynthesis | 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein] | - |
? | |
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate | Streptomyces griseus IFO13350 | AfsA is the key enzyme for A factor biosynthesis | 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | P18394 | - |
- |
Streptomyces griseus IFO13350 | P18394 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate | - |
Streptomyces griseus | 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein] | - |
? | |
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate | AfsA is the key enzyme for A factor biosynthesis | Streptomyces griseus | 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein] | - |
? | |
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate | - |
Streptomyces griseus IFO13350 | 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein] | - |
? | |
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate | AfsA is the key enzyme for A factor biosynthesis | Streptomyces griseus IFO13350 | 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37700, SDS-PAGE | Streptomyces griseus |
Synonyms | Comment | Organism |
---|---|---|
afsA | - |
Streptomyces griseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
- |
Streptomyces griseus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 30 | at 30°C, the activity decreases to 32% of that measured at 15°C, and almost no activity is detected at 35°C | Streptomyces griseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
glycerone phosphate | pH 7.5, 15°C | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | Trisx01HCl buffer | Streptomyces griseus |
7 | 8 | sodium phosphate-citrate buffer | Streptomyces griseus |