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Literature summary for 2.3.1.230 extracted from

  • Drees, S.; Li, C.; Prasetya, F.; Saleem, M.; Dreveny, I.; Williams, P.; Hennecke, U.; Emsley, J.; Fetzner, S.
    PqsBC, a condensing enzyme in the biosynthesis of the pseudomonas aeruginosa quinolone signal Crystal structure, inhibition, and reaction mechanism (2016), J. Biol. Chem., 291, 6610-6624 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.230

Application

Application Comment Organism
drug development the enzyme PqsBC is a potential drug target Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
the pqsB and pqsC genes of are cloned into plasmid pET28b and expresssed in Escherichia coli Rosetta2(DE3) Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, PqsBC(C129A) crystallized in space group P2(1)2(1)2(1) and the structure is determined to 2 A resolution, revealing that PqsB and PqsC have a pseudo-2-fold symmetry Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
octanoyl-CoA + (2-aminobenzoyl)acetate Pseudomonas aeruginosa
-
 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
octanoyl-CoA + (2-aminobenzoyl)acetate Pseudomonas aeruginosa ATCC 15692
-
 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I4X2 AND Q9I4X1 Q9I4X2: 2-heptyl-4(1H)-quinolone synthase subunit PqsB, Q9I4X1: 2-heptyl-4(1H)-quinolone synthase subunit PqsC
-
Pseudomonas aeruginosa ATCC 15692 Q9I4X2 AND Q9I4X1 Q9I4X2: 2-heptyl-4(1H)-quinolone synthase subunit PqsB, Q9I4X1: 2-heptyl-4(1H)-quinolone synthase subunit PqsC
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
octanoyl-CoA + (2-aminobenzoyl)acetate
-
 Pseudomonas aeruginosa 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
octanoyl-CoA + (2-aminobenzoyl)acetate
-
 Pseudomonas aeruginosa ATCC 15692 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?

Subunits

Subunits Comment Organism
heterodimer
-
 Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
PqsBC
-
 Pseudomonas aeruginosa

General Information

General Information Comment Organism
metabolism PqsBC, a key enzyme in the biosynthesis of the quorum-sensing signal molecules 2-heptyl-4(1H)-quinolone and Pseudomonas quinolone signal Pseudomonas aeruginosa