Cloned (Comment) | Organism |
---|---|
functional overexpression of His-tagged LasIDELTAG and point mutants in Escherichia coli as soluble proteins | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant detagged LasIDELTAG, hanging drop vapour diffusion method, from 1.5 M ammonium sulfate, 0.125 M disodium sulfate, and 0.1 M MOPS, pH 6.5, cryoprotection with 15% glycerol, heavy atom derivatization with Hg2+, single isomorphous replacement with anomalous scattering, X-ray diffraction structure determination and analysis at 2.3-3.1 A resolution, structure modeling | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
K150E/R154QE | site-directed mutagenesis, the mutant shows highly reduced activity compared to the fully active mutant LasIDELTAG | Pseudomonas aeruginosa |
K150Q | site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG | Pseudomonas aeruginosa |
K150Q/R154Q | site-directed mutagenesis, the mutant shows reduced activity compared to the fully active mutant LasIDELTAG | Pseudomonas aeruginosa |
R154E | site-directed mutagenesis, the mutant shows reduced activity compared to the fully active mutant LasIDELTAG | Pseudomonas aeruginosa |
R154Q | site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG | Pseudomonas aeruginosa |
R172A | site-directed mutagenesis, the mutant shows increased activity compared to the fully active mutant LasIDELTAG | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | LasI and LasR form a quorum-sensing system playing a pivotal role in virulence gene regulation of the opportunistic human pathogen Pseudomonas aeruginosa | ? | - |
? | |
S-adenosyl-L-methionine + 3-oxododecanoyl-[acyl-carrier protein] | Pseudomonas aeruginosa | substrate of LasI | 5'-methylthioadenosine + N-3-oxododecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene lasI | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged LasIDELTAG from Escherichia coli by nickel affinity chromatography and gel filtration, the His-tag is removed by thrombin cleavage | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone | substrate binding structure | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of mutant LasI enzymes | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | LasI and LasR form a quorum-sensing system playing a pivotal role in virulence gene regulation of the opportunistic human pathogen Pseudomonas aeruginosa | Pseudomonas aeruginosa | ? | - |
? | |
S-adenosyl-L-methionine + 3-oxododecanoyl-[acyl-carrier protein] | substrate of LasI | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-3-oxododecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acyl-homoserinelactone synthase | - |
Pseudomonas aeruginosa |
AHL synthase | - |
Pseudomonas aeruginosa |
LasI | - |
Pseudomonas aeruginosa |
LasR | - |
Pseudomonas aeruginosa |