Application | Comment | Organism |
---|---|---|
drug development | the enzyme is an antibiotic target in Gram-positive bacteria and might also be a target in Gram-negative bacteria | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
gene fabF, recombinant wild-type and mutant N-terminally His6-tagged enzyme expressions from vector pNIC28-Bsa4 including a TEV cleavage site in Escherichia coli strain BL21(DE3)pLysS | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant free wild-type enzyme, and mutant C164Q enzyme, free or in complex with 3-(benzoylamino)-2-hydroxybenzoic acid, mixing of 0.001 ml of 20 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 150 mM NaCl, with 0.001 ml reservoir solution containing 0.2 M MgCl2, 0.1 M Tris-HCl, pH 7.0, 10% w/v PEG 8000 for the wild-type enzyme, or 0.001 ml of 20 mg/ml protein in 50 mM Na2HPO4, pH 7.8, 150 mM NaCl, 10% v/v glycerol, and 0.5 mM DTT with 0.001 ml of reservoir consisting of 0.2 M NH4HCO2, 25% w/v PEG 3350 for the mutant enzyme, equilibration against 0.06 ml reservoir solution, at 20°C, X-ray diffraction structure determination and analysis at 1.67-2.46 A resolution, molecular replacement | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C164Q | site-directed mutagenesis, a mutant in which the binding site is altered to resemble the substrate-bound state | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(benzoylamino)-2-hydroxybenzoic acid | binds outside the active site of the enzyme, binding structure with wild-type and C164Q mutant enzymes, overview. Access to the depths of the active site of the PaFabF apoenzyme is restricted by the conformations of Phe230 and Phe400. 3-(benzoylamino)-2-hydroxybenzoic acid/Mg2+ ion pair selectively binds into and perhaps contributes to the formation of a stable binding site on the surface of the enzyme distant from the active site, from which it is likely to be occluded by steric hindrance | Pseudomonas aeruginosa | |
platensimycin | a natural product inhibitor | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
recombinant enzyme, gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | G3XDA2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinit chromatography, tag cleavage through TEV, dialysis, and gel filtration | Pseudomonas aeruginosa |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
3-oxoacyl-ACP synthase II | - |
Pseudomonas aeruginosa |
beta-ketoacyl-(acyl-carrier-protein) synthase II | - |
Pseudomonas aeruginosa |
FabF | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in fatty-acid biosynthesis | Pseudomonas aeruginosa |
additional information | active site structure of wild-type and mutant enzymes, ligand binding structures, overview | Pseudomonas aeruginosa |
physiological function | the enzyme is involved in fatty-acid biosynthesis | Pseudomonas aeruginosa |