Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant His-tagged FabF in Escherichia coli strain BL21(DE3) | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant mutant H303A from 20% polyethylene glycol 3350, 0.2 M potassium acetate, X-ray diffraction structure determination and analysis | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
C164A | site-directed mutagenesis, inactive mutant | Streptococcus pneumoniae |
C164A/H337A | site-directed mutagenesis, inactive mutant | Streptococcus pneumoniae |
C164A/K332A | site-directed mutagenesis, inactive mutant | Streptococcus pneumoniae |
E346A | site-directed mutagenesis, the mutant shows similar activity compared to the wild-type enzyme | Streptococcus pneumoniae |
E396A | site-directed mutagenesis, the mutant shows no condensation activity but retains about 50% of wild-type transacylation activity with acyl-ACP and ACP, and 40% of wild-type decarboxylation activity | Streptococcus pneumoniae |
H303A | site-directed mutagenesis, the mutant shows 74% reduced condensation activity, 40% reduced transacylation activity, and 5fold increased decarboxylation activity, compared to the wild-type enzyme | Streptococcus pneumoniae |
H337A | site-directed mutagenesis, inactive mutant | Streptococcus pneumoniae |
K332A | site-directed mutagenesis, the mutant shows no condensation activity but retains about 30% of wild-type transacylation activity with acyl-ACP and ACP, and 10% of wild-type decarboxylation activity | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Streptococcus pneumoniae | - |
? + CO2 + [acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant His-tagged FabF from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Streptococcus pneumoniae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | elongation condensing enzyme, catalytic mechanism involving Cys134, His337, and His303, forming the catalytic triad, as well as Phe396, and a water molecule bound to the active site, analysis of residues involved in the different reaction steps, overview | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | analysis of interaction between FabF and the acyl-carrier protein | Streptococcus pneumoniae | ? | - |
? | |
myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Streptococcus pneumoniae | ? + CO2 + [acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-ACP synthase II | - |
Streptococcus pneumoniae |
FabF | - |
Streptococcus pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptococcus pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7 | assay at | Streptococcus pneumoniae |