Cloned (Comment) | Organism |
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expression of a 49 kDa fragment containing residues 311-729 of the intact enzyme and a C-terminal His tag, in Escherichia coli | Moorella thermoacetica |
Crystallization (Comment) | Organism |
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49 kDa fragment containing residues 311-729 of the intact enzym. In the fragment, domains A2 and A3 have significantlymoved to each other, corresponding to a rotation around a hinge region located close to the C-terminus of the long interdomain helix | Moorella thermoacetica |
crystal structure of recombinant ACS lacking the N-terminal domain that interacts with carbon monoxide dehydrogenase shows a large reorganization of the remaining two globular domains, producing a narrow cleft of suitable size, shape, and nature to bind CoA. Sequence comparisons with homologous archaeal enzymes that naturally lack the N-terminal domain show that many amino acids lining this cleft are conserved. Besides the typical [4Fe-4S] center, the A-cluster contains only one proximal metal ion that is most likely Cu or Zn. Incorporation of a functional Ni2Fe4S4 A-cluster would require only minor structural rearrangements | Moorella thermoacetica |
Organism | UniProt | Comment | Textmining |
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Moorella thermoacetica | - |
- |
- |
Moorella thermoacetica | P27988 | - |
- |