Cloned (Comment) | Organism |
---|---|
gene agcB, expression as His-tagged protein in Escherichia coli strain BL21(DE3) | Enterococcus faecalis |
Crystallization (Comment) | Organism |
---|---|
purified His-tagged wild-type PTCase, from a solution containing 200 mM magnesium sulfate, 15-17% w/v PEG 3350, 100 mM Bis-tris, pH 5.5, soaking in mother liquor containing 25% v/v ethylene glycol for 1 min for cryoprotection, X-ray diffraction structure determination and analysis at 3.2 A resolution | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | in contrast to other trimeric carbamoyltransferases, PTCase binds both carbamoyl phosphate and putrescine with Hill coefficients at saturating concentrations of the other substrate of 1.53 and 1.80, respectively | Enterococcus faecalis | |
0.101 | - |
Carbamoyl phosphate | pH and temperature not specified in the publication | Enterococcus faecalis | |
0.136 | - |
putrescine | pH and temperature not specified in the publication | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + putrescine | Enterococcus faecalis | - |
phosphate + N-carbamoylputrescine | - |
? | |
carbamoyl phosphate + putrescine | Enterococcus faecalis ATCC 700802 | - |
phosphate + N-carbamoylputrescine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | Q837U7 | gene agcB | - |
Enterococcus faecalis ATCC 700802 | Q837U7 | gene agcB | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + putrescine | - |
Enterococcus faecalis | phosphate + N-carbamoylputrescine | - |
? | |
carbamoyl phosphate + putrescine | substrate binding structures, overview | Enterococcus faecalis | phosphate + N-carbamoylputrescine | - |
? | |
carbamoyl phosphate + putrescine | - |
Enterococcus faecalis ATCC 700802 | phosphate + N-carbamoylputrescine | - |
? | |
carbamoyl phosphate + putrescine | substrate binding structures, overview | Enterococcus faecalis ATCC 700802 | phosphate + N-carbamoylputrescine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure comparison, overview | Enterococcus faecalis |
trimer | PTCase also has a unique structural feature: a long C-terminal helix that interacts with the adjacent subunit to enhance intersubunit interactions in the molecular trimer. The C-terminal helix os essential for both formation of the functional trimer and catalytic activity, since truncated PTCase without the C-terminal helix aggregates and has only 3% of native catalytic activity | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
PTCase | - |
Enterococcus faecalis |
General Information | Comment | Organism |
---|---|---|
additional information | the C-terminal helix is essential for both formation of the functional trimer and catalytic activity, since truncated PTCase without the C-terminal helix aggregates and has only 3% of native catalytic activity. Active site structure, overview | Enterococcus faecalis |