Cloned (Comment) | Organism |
---|---|
M.HhaI overexpression in Escherichia coli strain ER1727 | Haemophilus parahaemolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(1H)-pyrimidinone riboside | i.e. zebularine, is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds. The ternary complexes between the enzyme, 2-(1H)-pyrimidinone inhibitor, and the cofactor S-adenosyl-L-methionine are maintained through the formation of a reversible covalent interaction | Haemophilus parahaemolyticus | |
5-azacytidine | zebularine is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds | Haemophilus parahaemolyticus | |
additional information | inhibitor binding kinetics | Haemophilus parahaemolyticus | |
oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone | oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone at the enzymatic target site are competitive inhibitors of both prokaryotic and mammalian DNA C5 methyltransferases, overview | Haemophilus parahaemolyticus | |
oligodeoxyribonucleotides containing 5-azacytidine | 5-azacytosine oligodeoxyribonucleotides form complexes with C5 DNA methyltransferases that are irreversible when the 5-azacytosine ring is intact, overview | Haemophilus parahaemolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | Haemophilus parahaemolyticus | - |
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus parahaemolyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | - |
Haemophilus parahaemolyticus | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA (cytosine-C5) methyltransferase | - |
Haemophilus parahaemolyticus |
M.HhaI | - |
Haemophilus parahaemolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Haemophilus parahaemolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Haemophilus parahaemolyticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | the ternary complexes between the enzyme, 2-(1H)-pyrimidinone inhibitor, and the cofactor S-adenosyl-L-methionine are maintained through the formation of a reversible covalent interaction | Haemophilus parahaemolyticus |