Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Pyrococcus abyssi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulating effect | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | - |
the genome of the hyperthermophilic archaeon Pyrococcus abyssi contains two open reading frames, PAB0719 and PAB0760,which are shownby comparative phylogenetic analyses to belong to the COG2265 m5U methyltransferases | - |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | recombinant PAB0719 is shown to function like TrmA from Escherichia coli and catalyzes m5U formation at position 54 in Pyrococcus abyssi tRNAs | Pyrococcus abyssi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAB0719 | - |
Pyrococcus abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
kinetics of m5U formation by PAB0760 proceed faster at 80°C than at 50°C | Pyrococcus abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pyrococcus abyssi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Pyrococcus abyssi |