Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Fluorouracil substituted tRNA | - |
Escherichia coli | |
NO2Ura-tRNA | potent mechanism-based inhibitor, competitive with respect to tRNA, can be reversed by dilution into a large excess of tRNA substrate, forms a reversible covalent complex in which Cys-324 of RUMT is linked to the 6-position of NO2-Ura 54 in NO2-Ura-tRNA, mechanism | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00037 | - |
uracil54 in yeast tRNA-Phe | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA | catalytic mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uracil54 in yeast tRNA-Phe | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA-Phe | - |
? | |
S-adenosyl-L-methionine + uracil54 in yeast tRNA1-Phe | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA1-Phe | - |
r | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: unmodified yeast tRNAPhe | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0317 | - |
uracil54 in yeast tRNA1-Phe | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
NO2Ura-tRNA | - |
Escherichia coli |