Cloned (Comment) | Organism |
---|---|
trmA gene encoding for RUMT is cloned into a pET vector and transformed into Escherichia coli BL21 (DE3) cells, extremely high-level expression system | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Fluorouracil substituted tRNA | first-order inactivation in the presence of S-adenosyl-L-methionine, potent mechanism-based irreversible inhibition, formation of a stable covalent complex containing enzyme, FUra-tRNA and the methyl group of AdoMet | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli GB-1-5-39 / pTN102 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
rapid purification scheme | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00165 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uridine54 in tRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop | ? | |
S-adenosyl-L-methionine + uridine54 in tRNA | - |
Escherichia coli GB-1-5-39 / pTN102 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay at room temperature | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |