Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethylthioadenosine | 0.2 mM | Escherichia coli | |
L-ethionine | selective inhibitor of uracil tRNA methylase, non-competitive to S-adenosyl-L-methionine | Escherichia coli | |
L-methionine | 30 mM, weak | Escherichia coli | |
additional information | not inhibited by alanine, valine, ethionine sulfoxide | Escherichia coli | |
norleucine | 30 mM, weak | Escherichia coli | |
S-adenosyl-L-ethionine | 0.6 mM | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli | specific inhibition of enzyme by ethionine in vivo | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strains B and M3S | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: thymine-deficient tRNA from E. coli trmA-mutant | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: thymine-deficient tRNA from E. coli | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | specific inhibition of enzyme by ethionine in vivo | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |