Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates | Escherichia coli | |
spermine | slight activation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by spermine, adenosine, L-homocysteine, snake venom phosphodiesterase | Escherichia coli | |
Ni2+ | - |
Escherichia coli | |
p-hydroxymercuribenzoate | - |
Escherichia coli | |
ribonuclease | inactivates | Escherichia coli | |
S-adenosyl-L-homocysteine | product inhibition, reversed by S-adenosylmethionine | Escherichia coli | |
Spleen phosphodiesterase | weak | Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
S-adenosyl-L-methionine | - |
Escherichia coli | |
0.29 | - |
uracil54 in methyl deficient tRNA from E. coli | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Escherichia coli | |
Mg2+ | in presence of Mg2+ 4fold increase of activity | Escherichia coli | |
Mn2+ | activates | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0064 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not as methyl group acceptors: methyl-deficient DNA from E. coli, polyadenylic acid, polycytidylic acid, polyuridylic acid, polyinosinic acid, copolymers of adenylic acid and uridylic acid and of guanylic acid and cytidylic acid, tobacco mosaic viral RNA, turnip yellow mosaic viral RNA, RNA from bacteriophage f2, CMP, AMP, UMP, GMP, rRNA | Escherichia coli | additional information | - |
? | |
S-adenosyl-L-methionine + uracil54 in methyl deficient tRNA from E. coli | - |
Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: methyl-deficient tRNA from E. coli | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
ir | |
S-adenosyl-L-methionine + uridine54 in tRNA | highly specific for tRNA and S-adenosyl-L-methionine, cannot be replaced by methionine plus ATP, formaldehyde plus tetrahydrofolic acid, methylcobalamin and methyltetrahydrofolic acid | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
ir | |
S-adenosyl-L-methionine + uridine54 in tRNA | extend of methylation is directly proportional to the amount of tRNA added, 0.5% of nucleotide residues are methylated | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
ir |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | triethanolamine buffer | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
S-adenosyl-L-homocysteine | - |
Escherichia coli |