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Literature summary for 2.1.1.33 extracted from

  • Tomikawa, C.; Takai, K.; Hori, H.
    Kinetic characterization of substrate-binding sites of thermostable tRNA methyltransferase (TrmB) (2018), J. Biochem., 163, 133-142 .
    View publication on PubMed
Search for more literature for 2.1.1.33

Protein Variants

Protein Variants Comment Organism
D133A complete loss of activity Aquifex aeolicus
D133N complete loss of activity Aquifex aeolicus
D74A 21% of wild-type activity Aquifex aeolicus
D97A 21% of wild-type activity Aquifex aeolicus
E168A 100% of wild-type activity Aquifex aeolicus
E47A 0.4% of wild-type activity Aquifex aeolicus
H104A 140% of wild-type activity Aquifex aeolicus
K101A 36% of wild-type activity Aquifex aeolicus
R107A 140% of wild-type activity Aquifex aeolicus
R108A 2% of wild-type activity Aquifex aeolicus
T132A 70% of wild-type activity Aquifex aeolicus
T132C 18% of wild-type activity Aquifex aeolicus
T132S 27% of wild-type activity Aquifex aeolicus
T165A 7% of wild-type activity Aquifex aeolicus
Y167A 0.2% of wild-type activity Aquifex aeolicus
Y167F 12% of wild-type activity Aquifex aeolicus
Y95A 2% of wild-type activity Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 S-adenosyl-L-methionine mutant D97A, 55°C, pH not specified in the publication Aquifex aeolicus
0.0007
-
 S-adenosyl-L-methionine wild-type, 55°C, pH not specified in the publication Aquifex aeolicus
0.0008
-
 S-adenosyl-L-methionine mutant D74A, 55°C, pH not specified in the publication Aquifex aeolicus
0.001
-
 S-adenosyl-L-methionine mutant T132S, 55°C, pH not specified in the publication Aquifex aeolicus
0.0017
-
 S-adenosyl-L-methionine mutant T132A, 55°C, pH not specified in the publication Aquifex aeolicus
0.0025
-
 S-adenosyl-L-methionine mutant H104A, 55°C, pH not specified in the publication Aquifex aeolicus
0.0025
-
 S-adenosyl-L-methionine mutant R107A, 55°C, pH not specified in the publication Aquifex aeolicus
0.003
-
 S-adenosyl-L-methionine mutant K101A, 55°C, pH not specified in the publication Aquifex aeolicus
0.003
-
 S-adenosyl-L-methionine mutant T132C, 55°C, pH not specified in the publication Aquifex aeolicus
0.005
-
 S-adenosyl-L-methionine mutant T165A, 55°C, pH not specified in the publication Aquifex aeolicus
0.006
-
 S-adenosyl-L-methionine mutant Y167F, 55°C, pH not specified in the publication Aquifex aeolicus
0.007
-
 S-adenosyl-L-methionine mutant E168A, 55°C, pH not specified in the publication Aquifex aeolicus
0.007
-
 S-adenosyl-L-methionine mutant E47A, 55°C, pH not specified in the publication Aquifex aeolicus
0.0075
-
 S-adenosyl-L-methionine mutant Y95A, 55°C, pH not specified in the publication Aquifex aeolicus
0.01
-
 S-adenosyl-L-methionine mutant R108A, 55°C, pH not specified in the publication Aquifex aeolicus
0.019
-
 S-adenosyl-L-methionine mutant Y167A, 55°C, pH not specified in the publication Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O66479
-
-

Purification (Commentary)

Purification (Comment) Organism
purification method to remove contaminating RNA completely by using 6 M urea Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine46 in tRNAPhe substrate tRNAPhe of Saccharomyces cerevisiae Aquifex aeolicus S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAPhe
-
 ?

Synonyms

Synonyms Comment Organism
TrmB
-
 Aquifex aeolicus

General Information

General Information Comment Organism
physiological function residues Glu47, Tyr95, Arg108, Thr165 and Tyr167 residues are important for AdoMet binding and Asp74, Asp97, and Thr132 are important for the methyltransfer reaction. During the reaction, the carboxyl group in Asp133 captures the proton of N-H in the guanine base. Thr132 may assist the interaction by formation of a hydrogen bond between the OH group in Thr132 and the O6 atom in the guanine base. Interactions between Thr132, Asp133 and tRNA decide the direction of the guanine base and the N7 atom in G46 causes the nucleophilic attack against the activated methyl group in the bound AdoMet Aquifex aeolicus