Literature summary for 2.1.1.295 extracted from

Tang, Y.; Fu, X.; Shen, Q.; Tang, K.
Roles of MPBQ-MT in promoting alpha/gamma-tocopherol production and photosynthesis under high light in lettuce (2016), PLoS ONE, 11, e0148490 .

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Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, the coding region is 1014bp long, divided into 3 exons by 2 introns, sequence comparisons, recombinant overexpression of the enzyme in Lactuca sativa leaves by Agrobacterium-mediated transformation using the pCAMBIA 2300 vector, quantitative PCR expression analysis showing that the transcription level of LsMT in transgenic plants increases by 1.5-5folds compared to that in wild-type plants. The transformants contain a 60%-93% higher level of gamma-tocopherol and 41%-93% higher level of alpha-tocopherol compred to wild-type. Overexpression of LsMT causes an elevation of plastoquinone level in lettuce	Lactuca sativa

Cloned (Comment)

Organism

DNA and amino acid sequence determination and analysis, the coding region is 1014bp long, divided into 3 exons by 2 introns, sequence comparisons, recombinant overexpression of the enzyme in Lactuca sativa leaves by Agrobacterium-mediated transformation using the pCAMBIA 2300 vector, quantitative PCR expression analysis showing that the transcription level of LsMT in transgenic plants increases by 1.5-5folds compared to that in wild-type plants. The transformants contain a 60%-93% higher level of gamma-tocopherol and 41%-93% higher level of alpha-tocopherol compred to wild-type. Overexpression of LsMT causes an elevation of plastoquinone level in lettuce

Lactuca sativa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	the LsMT sequence is predicted to have an N-terminal chloroplast transit peptide (cTP) of 52 residues	Lactuca sativa	9507	-

Organism

Organism	UniProt	Comment	Textmining
Lactuca sativa	D2D1G3	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Lactuca sativa	-

Synonyms

Synonyms	Comment	Organism
2-methyl-6-phytyl-1, 4-benzoquinol methyltransferase	-	Lactuca sativa
LSMT	-	Lactuca sativa
MPBQ-MT	-	Lactuca sativa
MPBQ/MSBQ transferase	UniProt	Lactuca sativa

General Information

General Information	Comment	Organism
metabolism	2-methyl-6-phytyl-1,4-benzoquinol methyltransferase (MPBQ-MT) is a vital enzyme catalyzing a key methylation step in both alpha/gamma-tocopherol and plastoquinone biosynthetic pathway	Lactuca sativa
physiological function	2-methyl-6-phytyl-1,4-benzoquinol methyltransferase (MPBQ-MT) is a vital enzyme catalyzing a key methylation step in both alpha/gamma-tocopherol and plastoquinone biosynthetic pathway. Overexpression of LsMT significantly increases plastoquinone (PQ) level in lettuce. The increase of tocopherol and plastoquinone levels by LsMT overexpression conduce to the improvement of plants' tolerance and photosynthesis under high light stress, by directing excessive light energy toward photosynthetic production rather than toward generation of more photooxidative damage. No significant difference in the expression levels of HPPD, HPT, TC and gamma-TMT is observed between wild-type and transgenic plants, indicating that overexpression of LsMT does not impact on the expression of other genes up- and downstream in the tocopherol biosynthetic pathway. Mechanism by which overexpression of MPBQ-MT leads to enhanced photosynthesis and tolerance to photodamage under high light in lettuce, overview	Lactuca sativa