Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) CodonPlus cells | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
apoenzyme and enzyme bound to S-adenosyl-L-methionine or S-adenosyl-L-homocysteine, sitting drop vapor diffusion method, using 200 mM ammonium citrate (pH 7.0), 10 mM HEPES (pH 7.5), 20% (w/v) PEG3,350, 20 mM sodium citrate, and 1% (v/v) 2-propanol or microseeding method using 200 mM ammonium citrate (pH 7.0) and 20% (w/v) PEG3350 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
R88A | inactive | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72430 | - |
2 * 72430, sedimentation velocity analytical ultracentrifugation | Saccharomyces cerevisiae |
80022 | - |
2 * 80022, calculated from amino acid sequence | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine + CO2 | Saccharomyces cerevisiae | - |
2 S-adenosyl-L-homocysteine + wybutosine | - |
? | |
2 S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine + CO2 | Saccharomyces cerevisiae BY4742 | - |
2 S-adenosyl-L-homocysteine + wybutosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q08282 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine + CO2 | - |
Saccharomyces cerevisiae | 2 S-adenosyl-L-homocysteine + wybutosine | - |
? | |
2 S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine + CO2 | - |
Saccharomyces cerevisiae BY4742 | 2 S-adenosyl-L-homocysteine + wybutosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 72430, sedimentation velocity analytical ultracentrifugation | Saccharomyces cerevisiae |
homodimer | 2 * 80022, calculated from amino acid sequence | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
tRNA-yW synthesizing enzyme-4 | - |
Saccharomyces cerevisiae |
TYW4 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | dependent on | Saccharomyces cerevisiae |