Application | Comment | Organism |
---|---|---|
analysis | ergot alkaloid pathway reconstruction in Aspergillus nidulans is an approach used to better understand the biosynthesis of these mycotoxins | Aspergillus fumigatus |
Cloned (Comment) | Organism |
---|---|
recombinant heterologous enzyme expression in Aspergillus nidulans | Aspergillus fumigatus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an engineered strain named Aspergillus nidulans WFC, expressing ergot alkaloid synthesis genes dmaW, easF, and easC, produces the established intermediate N-methyl-4-dimethylallyltryptophan, as well as an ergot alkaloid, that is characterized as ergotryptamine, it differs from N-methyl-4-dimethylallyltryptophan by the loss of the carboxyl group, addition of a hydroxyl group, and shift in position of a carbon-carbon double bond, by mass spectrometry, labeling, and NMR. Feeding studies with Aspergillus mutants do not show ergotryptamine turnover, suggesting it is a pathway byproduct as opposed to an authentic intermediate | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan | Aspergillus fumigatus | - |
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan | - |
Aspergillus fumigatus | S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Aspergillus fumigatus |