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Literature summary for 2.1.1.202 extracted from

  • Yang, T.; Low, J.J.A.; Woon, E.C.Y.
    A general strategy exploiting m5C duplex-remodelling effect for selective detection of RNA and DNA m5C methyltransferase activity in cells (2020), Nucleic Acids Res., 48, e5 .
    View publication on PubMed
Search for more literature for 2.1.1.202

Inhibitors

Inhibitors Comment Organism Structure
additional information cell-based screening of NSUN2 inhibitors Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetic analyses of the methylation of m5Cprobe, tRNA and ssRNA by NSUN2 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O14717
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-
additional information NSUN2 is overexpressed in a number of human cancers Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + CpG-rich ssRNA (5'-CGCGCGCGCGCG-3') m5C methylation directly alters the phosphate backbone in CpG RNA duplex, leading to a C3'-endo to C2'-endo sugar pucker switch of the terminal residues under physiologically-relevant conditions. m5C triggers a B-to-Z DNA, but not A-to-Z, transformation in CpG DNA duplex. The m5C-probe has the sensitivity to detect a single m5C mark change Homo sapiens ?
-
 ?
S-adenosyl-L-methionine + cytosine in tRNALeu(CAA)
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine in tRNALeu(CAA)
-
 ?

Synonyms

Synonyms Comment Organism
NOP2/Sun RNA methyltransferase 2
-
 Homo sapiens
NSUN2
-
 Homo sapiens
TRDMT1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism m5C methylation of RNA is catalysed by the NOL1/NOP2/Sun domain (NSUN) RNA methyltransferase family, which includes NSUN1-7, as well as the DNA MTase homologue TRDMT1 (formerly DNMT2) Homo sapiens
additional information development of m5C-responsive probes, as a strategy for discriminating RNA and DNA m5C methyltransferase activity in cells (cf. EC 2.1.1.37), i.e. the m5C-switchable probe strategy, method development and evaluation, overview. The m5C-probe contains a 5'-terminal fluorescent nucleotide PC (2'-O-methyl 6-phenylpyrrolocytidine), which lights up spontaneously in response to m5C-induced terminal sugar pucker switch. When the probe is unmethylated, pC is able to base-pair with guanine in the complementary strand and stack strongly with its adjacent base. This results in efficient quenching of pC fluorescence through photoinduced electron transfer. m5C methylation of the probe by RNA:m5C MTase (e.g. NSUN2), however, is expected to trigger a C2'-endo to C3'-endo sugar pucker switch in PC and, since the sugar ring pucker defines the glycosidic bond angle, such a change in sugar puckering will also convert the orientation of PC base from axial to equatorial. This, in turn, disrupts its base-pairing and base-stacking interactions, leading to fluorescence activation. Schematic representation of 2'-OMe RNA probes and their methylated counterparts. The composition of the probes is confirmed through MALDI-TOF mass spectrometric analysis Homo sapiens