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Literature summary for 2.1.1.20 extracted from

  • Swiderek, K.; Tunon, I.; Williams,I. H.; Moliner, V.
    Insights on the origin of catalysis on glycine N-methyltransferase from computational modeling (2018), J. Am. Chem. Soc., 140, 4327-4334 .
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
Y21A comparison of QM/MM kinetic data for the methyl transfer among wild-type and mutants. Wild-type protein generates the most favorable electrostatic environment to stabilize the charge developed on the methyl group in the transition state, and thus is the most favourable environment to catalyze the reaction. The detrimental effect of substitution of Tyr21 on the electrostatic potential is partially compensated by His142 that, by approaching to the methyl group, generates a higher potential Rattus norvegicus
Y21F comparison of QM/MM kinetic data for the methyl transfer among wild-type and mutants. Wild-type protein generates the most favorable electrostatic environment to stabilize the charge developed on the methyl group in the transition state, and thus is the most favourable environment to catalyze the reaction. The detrimental effect of substitution of Tyr21 on the electrostatic potential is partially compensated by His142 that, by approaching to the methyl group, generates a higher potential Rattus norvegicus
Y21G comparison of QM/MM kinetic data for the methyl transfer among wild-type and mutants. Wild-type protein generates the most favorable electrostatic environment to stabilize the charge developed on the methyl group in the transition state, and thus is the most favourable environment to catalyze the reaction. The detrimental effect of substitution of Tyr21 on the electrostatic potential is partially compensated by His142 that, by approaching to the methyl group, generates a higher potential Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P13255
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Source Tissue

Source Tissue Comment Organism Textmining
liver
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 Rattus norvegicus
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General Information

General Information Comment Organism
physiological function QM/MM kinetic ananlysis. The methyl-group charge reaches a maximum after passing the transition state and decreases again. The donor-acceptor distance reaches the minimum value at this point, and the averaged electrostatic potentials created by the different environments at the S donor, N acceptor and C methyl atoms change from the reactant state to the point with a value of the reaction coordinate around +0.5 A and it remain stable from this point on in all environments Rattus norvegicus