Protein Variants | Comment | Organism |
---|---|---|
D124A | the mutant D124A is unable to rescue the growth defect of the rrmJ deletion strain, indicating that this mutation causes the inactivation of RrmJ in vivo | Escherichia coli |
D83A | the mutant D83A is unable to rescue the growth defect of the rrmJ deletion strain, indicating that this mutation causes the inactivation of RrmJ in vivo | Escherichia coli |
E199A | the RrmJ deletion strains expressing the E199A variant protein shows only slight growth defects, indicating that the residue is not as important in the catalytic mechanism | Escherichia coli |
K164A | the mutant D83A is unable to rescue the growth defect of the rrmJ deletion strain, indicating that this mutation causes the inactivation of RrmJ in vivo | Escherichia coli |
K38A | the mutant D83A is unable to rescue the growth defect of the rrmJ deletion strain, indicating that this mutation causes the inactivation of RrmJ in vivo | Escherichia coli |
Y201A | the RrmJ deletion strains expressing the Y201A variant protein shows only slight growth defects, indicating that the residue is not as important in the catalytic mechanism | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | apparent Km values for 50S ribosomal subunits. The apparent Km value for 23S rRNA within 50S ribosomal subunits is 0.0008 mM at 37°C | Escherichia coli | |
0.0037 | - |
S-adenosyl-L-methionine | pH 7.5, 37°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uridine2552 in 23S rRNA | Escherichia coli | the 2'-O-ribose methylation of the universally conserved base U2552 in the A-loop of the 23 S rRNA | S-adenosyl-L-homocysteine + 2'-O-methyluridine2552 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | A0A140N5X9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + uridine2552 in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine2552 in 23S rRNA | a reaction mechanism for the methyltransfer activity of RrmJ is proposed | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uridine2552 in 23S rRNA | the 2'-O-ribose methylation of the universally conserved base U2552 in the A-loop of the 23 S rRNA | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methyluridine2552 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + uridine2552 in 23S rRNA | the 2'-O-ribose methylation of the universally conserved base U2552 in the A-loop of the 23 S rRNA. The active site of RrmJ appears to be formed by a catalytic triad consisting of two lysine residues, Lys-38 and Lys-164, and the negatively charged residue Asp-124. Another highly conserved residue, Glu-199, that is present in the active site of RrmJ and VP39 appears to play only a minor role in the methyltransfer reaction in vivo. A reaction mechanism for the methyltransfer activity of RrmJ is proposed | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methyluridine2552 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FTSJ | - |
Escherichia coli |
heat shock protein RrmJ | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | 50°C: about 75% of maximal activity, 60°C: about 95% of maximal activity | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
RrmJ is a thermostable heat shock protein | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the apparent kcat value for 23 S rRNA within 50S ribosomal subunits is 0.001/sec mM at 37°C | Escherichia coli | |
0.001 | - |
S-adenosyl-L-methionine | pH 7.5, 37°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | absence of this methylation, which occurs late in the maturation process of the ribosome, appears to cause the destabilization and premature dissociation of the 50 S ribosomal subunit | Escherichia coli |