Literature summary for 2.1.1.104 extracted from

Xu, R.X.; Zhao, Y.; Gao, S.; Zhang, Y.Y.; Li, D.D.; Lou, H.X.; Cheng, A.X.
Functional characterization of a plastidal cation-dependent O-methyltransferase from the liverwort Plagiochasma appendiculatum (2015), Phytochemistry, 118, 33-41 .

Search for more literature for 2.1.1.104

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as GFP fusion protein in Escherichia coli and Nicotiana tabacum	Plagiochasma appendiculatum

Protein Variants

Protein Variants	Comment	Organism
L110A	the mutant shows about 9% of wild type activity with caffeic acid as substrate	Plagiochasma appendiculatum
L110G	the mutant shows about 9% of wild type activity with caffeic acid as substrate	Plagiochasma appendiculatum
L110S	the mutant shows about 9% of wild type activity with caffeic acid as substrate	Plagiochasma appendiculatum
L110V	the mutant shows about 29% of wild type activity with caffeic acid as substrate	Plagiochasma appendiculatum
L110Y	the mutant shows about 77% of wild type activity with caffeic acid as substrate	Plagiochasma appendiculatum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04388	-	quercetin	at pH 7.5 and 37°C	Plagiochasma appendiculatum
0.08382	-	eriodictyol	at pH 7.5 and 37°C	Plagiochasma appendiculatum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plastid	-	Plagiochasma appendiculatum	9536	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity	Plagiochasma appendiculatum

Organism

Organism	UniProt	Comment	Textmining
Plagiochasma appendiculatum	A0A0U2YR01	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel column chromatography	Plagiochasma appendiculatum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00271	-	with coniferyl alcohol as substrate, at pH 7.5 and 37°C	Plagiochasma appendiculatum
0.00443	-	with esculetin as substrate, at pH 7.5 and 37°C	Plagiochasma appendiculatum
0.00569	-	with coniferaldehyde as substrate, at pH 7.5 and 37°C	Plagiochasma appendiculatum
0.00705	-	with caffeoyl aldehyde as substrate, at pH 7.5 and 37°C	Plagiochasma appendiculatum
0.00897	-	with caffeoyl-CoA as substrate, at pH 7.5 and 37°C	Plagiochasma appendiculatum
0.01607	-	with caffeoyl alcohol as substrate, at pH 7.5 and 37°C	Plagiochasma appendiculatum
0.01923	-	with ferulic acid as substrate, at pH 7.5 and 37°C	Plagiochasma appendiculatum
0.02016	-	with caffeic acid as substrate, at pH 7.5 and 37°C	Plagiochasma appendiculatum
0.04714	-	with eriodictyol as substrate, at pH 7.5 and 37°C	Plagiochasma appendiculatum
0.05666	-	with luteolin as substrate, at pH 7.5 and 37°C	Plagiochasma appendiculatum
0.12782	-	with quercetin as substrate, at pH 7.5 and 37°C	Plagiochasma appendiculatum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	no activity with kaempferol and dihydrokaempferol	Plagiochasma appendiculatum	?	-	-
S-adenosyl-L-methionine + caffeic acid	-	Plagiochasma appendiculatum	S-adenosyl-L-homocysteine + ferulic acid	-	?
S-adenosyl-L-methionine + caffeoyl alcohol	-	Plagiochasma appendiculatum	S-adenosyl-L-homocysteine + coniferyl alcohol	-	?
S-adenosyl-L-methionine + caffeoyl aldehyde	-	Plagiochasma appendiculatum	S-adenosyl-L-homocysteine + coniferaldehyde	-	?
S-adenosyl-L-methionine + caffeoyl-CoA	-	Plagiochasma appendiculatum	S-adenosyl-L-homocysteine + feruloyl-CoA	-	?
S-adenosyl-L-methionine + coniferaldehyde	-	Plagiochasma appendiculatum	S-adenosyl-L-homocysteine + sinapaldehyde	-	?
S-adenosyl-L-methionine + coniferyl alcohol	-	Plagiochasma appendiculatum	S-adenosyl-L-homocysteine + sinapyl alcohol	-	?
S-adenosyl-L-methionine + eriodictyol	-	Plagiochasma appendiculatum	S-adenosyl-L-homocysteine + homoeriodictyol + hesperetin	-	?
S-adenosyl-L-methionine + esculetin	-	Plagiochasma appendiculatum	S-adenosyl-L-homocysteine + scopoletin	-	?
S-adenosyl-L-methionine + ferulic acid	-	Plagiochasma appendiculatum	S-adenosyl-L-homocysteine + sinapic acid	-	?
S-adenosyl-L-methionine + luteolin	-	Plagiochasma appendiculatum	S-adenosyl-L-homocysteine + chrysoeriol	-	?
S-adenosyl-L-methionine + quercetin	best substrate	Plagiochasma appendiculatum	S-adenosyl-L-homocysteine + isorhamnetin	-	?

Subunits

Subunits	Comment	Organism
?	x * 33710, calculated from amino acid sequence	Plagiochasma appendiculatum
?	x * 54000, His-tagged enzyme, SDS-PAGE	Plagiochasma appendiculatum

Synonyms

Synonyms	Comment	Organism
CCoAOMT	-	Plagiochasma appendiculatum
OMT1	-	Plagiochasma appendiculatum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.067	-	eriodictyol	at pH 7.5 and 37°C	Plagiochasma appendiculatum
0.156	-	quercetin	at pH 7.5 and 37°C	Plagiochasma appendiculatum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Plagiochasma appendiculatum	calculated from amino acid sequence	-	5.4

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.794	-	eriodictyol	at pH 7.5 and 37°C	Plagiochasma appendiculatum
3.552	-	quercetin	at pH 7.5 and 37°C	Plagiochasma appendiculatum

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Release 2023.1 (January 2023)