Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.5.3 extracted from

  • Dhouib, R.; Nasreen, M.; Othman, D.S.M.P.; Ellis, D.; Lee, S.; Essilfie, A.T.; Hansbro, P.M.; McEwan, A.G.; Kappler, U.
    The DmsABC sulfoxide reductase supports virulence in non-typeable Haemophilus influenzae (2021), Front. Microbiol., 12, 686833 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.5.3

Cloned(Commentary)

Cloned (Comment) Organism
co-transcription of dmsABCDE genes, quantitative RT-PCR enzyme expression analysis Haemophilus influenzae

Protein Variants

Protein Variants Comment Organism
additional information a Hi2019DELTAdmsA strain does not show any defects in anaerobic growth on chemically defined medium (CDM), and viability is also unaffected. Although Hi2019DELTAdmsA exhibits increased biofilm formation in vitro and greater resistance to hypochlorite killing compared to the isogenic wild-type strain, its survival in contact with primary human neutrophils, in infections of cultured tissue cells, or in a mouse model of lung infection is reduced compared to wild-type, Hi2019WT. The tissue cell infection model reveals a 2fold decrease in intracellular survival, while in the mouse model of lung infection Hi2019DELTAdmsA is strongly attenuated and below detection levels at 48 h post-inoculation. While Hi2019WT is recovered in approximately equal numbers from bronchoalveolar lavage fluid (BALF) and lung tissue, survival of Hi2019DELTAdmsA is reduced in lung tissue compared to BALF samples, indicating that Hi2019DELTAdmsA has reduced access to or survival in the intracellular niche Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylsulfoxide + menaquinol Haemophilus influenzae
-
 dimethylsulfide + menaquinone + H2O
-
 ?
dimethylsulfoxide + menaquinol Haemophilus influenzae RD
-
 dimethylsulfide + menaquinone + H2O
-
 ?
dimethylsulfoxide + menaquinol Haemophilus influenzae DSM 11121
-
 dimethylsulfide + menaquinone + H2O
-
 ?
dimethylsulfoxide + menaquinol Haemophilus influenzae KW20
-
 dimethylsulfide + menaquinone + H2O
-
 ?
dimethylsulfoxide + menaquinol Haemophilus influenzae ATCC 51907
-
 dimethylsulfide + menaquinone + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae P45004
-
-
Haemophilus influenzae ATCC 51907 P45004
-
-
Haemophilus influenzae DSM 11121 P45004
-
-
Haemophilus influenzae KW20 P45004
-
-
Haemophilus influenzae RD P45004
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylsulfoxide + menaquinol
-
 Haemophilus influenzae dimethylsulfide + menaquinone + H2O
-
 ?
dimethylsulfoxide + menaquinol
-
 Haemophilus influenzae RD dimethylsulfide + menaquinone + H2O
-
 ?
dimethylsulfoxide + menaquinol
-
 Haemophilus influenzae DSM 11121 dimethylsulfide + menaquinone + H2O
-
 ?
dimethylsulfoxide + menaquinol
-
 Haemophilus influenzae KW20 dimethylsulfide + menaquinone + H2O
-
 ?
dimethylsulfoxide + menaquinol
-
 Haemophilus influenzae ATCC 51907 dimethylsulfide + menaquinone + H2O
-
 ?
DL-methionine sulfoxide + menaquinol
-
 Haemophilus influenzae DL-methionine sulfide + menaquinone + H2O
-
 ?
DL-methionine sulfoxide + menaquinol
-
 Haemophilus influenzae RD DL-methionine sulfide + menaquinone + H2O
-
 ?
DL-methionine sulfoxide + menaquinol
-
 Haemophilus influenzae DSM 11121 DL-methionine sulfide + menaquinone + H2O
-
 ?
DL-methionine sulfoxide + menaquinol
-
 Haemophilus influenzae KW20 DL-methionine sulfide + menaquinone + H2O
-
 ?
DL-methionine sulfoxide + menaquinol
-
 Haemophilus influenzae ATCC 51907 DL-methionine sulfide + menaquinone + H2O
-
 ?
S-biotin sulfoxide + menaquinol
-
 Haemophilus influenzae S-biotin sulfide + menaquinone + H2O
-
 ?
S-biotin sulfoxide + menaquinol
-
 Haemophilus influenzae RD S-biotin sulfide + menaquinone + H2O
-
 ?
S-biotin sulfoxide + menaquinol
-
 Haemophilus influenzae DSM 11121 S-biotin sulfide + menaquinone + H2O
-
 ?
S-biotin sulfoxide + menaquinol
-
 Haemophilus influenzae KW20 S-biotin sulfide + menaquinone + H2O
-
 ?
S-biotin sulfoxide + menaquinol
-
 Haemophilus influenzae ATCC 51907 S-biotin sulfide + menaquinone + H2O
-
 ?
trimethylamine-N-oxide + menaquinol
-
 Haemophilus influenzae ? + menaquinone + H2O
-
 ?
trimethylamine-N-oxide + menaquinol
-
 Haemophilus influenzae RD ? + menaquinone + H2O
-
 ?
trimethylamine-N-oxide + menaquinol
-
 Haemophilus influenzae DSM 11121 ? + menaquinone + H2O
-
 ?
trimethylamine-N-oxide + menaquinol
-
 Haemophilus influenzae KW20 ? + menaquinone + H2O
-
 ?
trimethylamine-N-oxide + menaquinol
-
 Haemophilus influenzae ATCC 51907 ? + menaquinone + H2O
-
 ?

Synonyms

Synonyms Comment Organism
DmsABC sulfoxide reductase
-
 Haemophilus influenzae

Expression

Organism Comment Expression
Haemophilus influenzae expression of the dmsABC operon increases with decreasing oxygen availability. DmsABC expression in Haemophilus influenzae is associated with anaerobiosis but shows no substrate-dependent induction up

General Information

General Information Comment Organism
malfunction loss of DmsABC reduces Haemophilus influenzae fitness in interactions with human bronchial epithelial cells and neutrophils. Mutant Hi2019DELTAdmsA shows an increase in biofilm formation and increased resistance to HOCl Haemophilus influenzae
additional information the DmsABC sulfoxide reductase is encoded by a five-gene operon, dmsABCDE that is completely conserved in genomes of Haemophilus influenzae strains. The dmsABC genes encode the structural components of the enzyme, namely, the catalytic subunit DmsA, an iron-sulfur cluster-containing electron transfer subunit, DmsB, and the membrane anchor subunit, DmsC. The dmsDE genes encode two chaperones Haemophilus influenzae
physiological function DmsABC, a putative dimethylsulfoxide (DMSO) reductase, is required for fitness of the respiratory pathogen Haemophilus influenzae (Hi) in different models of infection. DmsABC sulfoxide reductase supports virulence in non-typeable Haemophilus influenzae. A role for DmsABC in Haemophilus influenzae infection, the conditions under which DmsABC is required in this bacterium are closely linked to interactions with the host. DmsABC is required for successful NTHi lung infection in mice. Under in vitro conditions, DmsABC is not required for Haemophilus influenzae viability Haemophilus influenzae