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Literature summary for 1.8.5.1 extracted from

  • Loi, M.; Leonardis, S.; Mulè, G.; Logrieco, A.; Paciolla, C.
    A novel and potentially multifaceted dehydroascorbate reductase increasing the antioxidant systems is induced by beauvericin in tomato (2020), Antioxidants (Basel), 9, 435 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.5.1

Inhibitors

Inhibitors Comment Organism Structure
beauvericin BEA, an ionophore, that induces oxidative stress, acts as an antimicrobial and as an enzyme inhibitor. Its cytotoxicity is mostly due to the selective ionophore (channel-forming) activity on biological membranes, which allows a flux of cations, particularly Ca2+, into the cell Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information GSH and DHA reduction follows a Michaelis-Menten kinetic Solanum lycopersicum
2
-
 dehydroascorbate pH 6.3, temperature not specified in the publication Solanum lycopersicum
12.71
-
 glutathione pH 6.3, temperature not specified in the publication Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Solanum lycopersicum 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 glutathione + dehydroascorbate Solanum lycopersicum
-
 glutathione disulfide + ascorbate
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum Q4VDN8 cv. Marmande
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from cytosolic fraction by centrifugation and dialysis Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
shoot
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + dehydroascorbate
-
 Solanum lycopersicum glutathione disulfide + ascorbate
-
 ?
additional information no activity with NADPH, alpha-lipoic acid, or DL-lipoamide as electron donors. The enzyme is GSH-dependent Solanum lycopersicum ?
-
-

Subunits

Subunits Comment Organism
monomer 1 * 26000, about, SDS-PAGE Solanum lycopersicum

Synonyms

Synonyms Comment Organism
dehydroascorbate reductase
-
 Solanum lycopersicum
DHAR
-
 Solanum lycopersicum
DHAR1
-
 Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
 assay at Solanum lycopersicum

Expression

Organism Comment Expression
Solanum lycopersicum beauvericin induces the enzyme up

General Information

General Information Comment Organism
metabolism the enzyme is part of the ascorbate-glutathione pathway, overview. This defense system is composed by enzymes such as the ascorbate peroxidase (APX, EC 1.11.1.11), the monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), the dehydroascorbate reductase (DHAR, EC 1.8.5.1), and the glutathione reductase (GR, EC 1.6.4.2), and compounds, such as ascorbate (ASC), dehydroascorbate (DHA), reduced (GSH) and oxidized (GSSG) glutathione Solanum lycopersicum
physiological function the enzyme is part of the ascorbate-glutathione pathway. This defense system is composed by enzymes such as the ascorbate peroxidase (APX, EC 1.11.1.11), the monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), the dehydroascorbate reductase (DHAR, EC 1.8.5.1), and the glutathione reductase (GR, EC 1.6.4.2), and compounds, such as ascorbate (ASC), dehydroascorbate (DHA), reduced (GSH) and oxidized (GSSG) glutathione. In this pathway, DHAR uses GSH to reduce DHA generated from the oxidation of ASC, thereby regenerating it. The enzyme plays a critical role in the ASC-GSH recycling reaction in higher plants Solanum lycopersicum