Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutathione | product inhibition | Setaria cervi | |
K+ | inhibitory in 0.1-1.0 M concentration range | Setaria cervi | |
Na+ | inhibitory in 0.1-1.0 M concentration range | Setaria cervi | |
NADP+ | product inhibition | Setaria cervi | |
NH4+ | inhibitory in 0.1-1.0 M concentration range | Setaria cervi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
NADPH | crude enzyme, at pH 7.0 and 22°C | Setaria cervi | |
0.021 | - |
NADPH | purified enzyme, at pH 7.0 and 22°C | Setaria cervi | |
0.03 | - |
NADP+ | purified enzyme at pH 7.0 and 22°C | Setaria cervi | |
0.0465 | - |
glutathione disulfide | crude enzyme, at pH 7.0 and 22°C | Setaria cervi | |
0.048 | - |
NADP+ | crude enzyme, at pH 7.0 and 22°C | Setaria cervi | |
0.05 | - |
glutathione disulfide | purified enzyme, at pH 7.0 and 22°C | Setaria cervi | |
5.9 | - |
glutathione | purified enzyme, at pH 7.0 and 22°C | Setaria cervi | |
6.8 | - |
glutathione | crude enzyme, at pH 7.0 and 22°C | Setaria cervi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, SDS-PAGE | Setaria cervi |
110000 | - |
gel filtration | Setaria cervi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH + H+ | Setaria cervi | - |
glutathione + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Setaria cervi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, ultrafiltration, CM-Sepharose column chromatography, DEAE-Sepharose column chromatography, GSHSepharose affinity column chromatography, and ADP-Sepharose affinity chromatography | Setaria cervi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0362 | - |
purified enzyme, at pH 7.0 and 22°C | Setaria cervi |
Storage Stability | Organism |
---|---|
0-4°C, 1 month, no appreciable effect of freezing and thawing on enzyme activity | Setaria cervi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH + H+ | - |
Setaria cervi | glutathione + NADP+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 55000, SDS-PAGE | Setaria cervi |
Synonyms | Comment | Organism |
---|---|---|
glutathione: NADP+ oxidoreductase | - |
Setaria cervi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
4 | there is no appreciable effect of freezing and thawing on enzyme activity for approximately 1 month | Setaria cervi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | broad pH optimum centered around pH 7.0 | Setaria cervi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Setaria cervi |