General Stability | Organism |
---|---|
the stability of the enzyme depends on the salt concentration. The maximum stability is found in the presence of 3.6 M NaCl | Haloferax mediterranei |
when the enzyme is dialysed against a 50 mM phosphate buffer, pH 7.0, containing 20% glycerol, the enzyme can be stored for 2 weeks without any significant loss of activity | Haloferax mediterranei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | - |
Haloferax mediterranei | |
azide | - |
Haloferax mediterranei | |
cyanide | - |
Haloferax mediterranei | |
additional information | potassium chlorate and dithiothreitol are not effective inhibitors | Haloferax mediterranei | |
sulfite | - |
Haloferax mediterranei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
reduced methyl viologen | pH 7.0, 60°C | Haloferax mediterranei | |
8.6 | - |
nitrite | pH 7.0, 60°C | Haloferax mediterranei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
1 * 66000 | Haloferax mediterranei |
74000 | - |
gel filtration | Haloferax mediterranei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + 6 reduced ferredoxin + 7 H+ | Haloferax mediterranei | assimilatory nitrite reductase | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? | |
nitrite + 6 reduced ferredoxin + 7 H+ | Haloferax mediterranei DSM 1411 | assimilatory nitrite reductase | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | - |
- |
- |
Haloferax mediterranei DSM 1411 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloferax mediterranei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.8 | - |
pH 7.0, 60°C | Haloferax mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with NADH as electron donor | Haloferax mediterranei | ? | - |
? | |
additional information | no activity with NADH as electron donor | Haloferax mediterranei DSM 1411 | ? | - |
? | |
nitrite + 6 reduced ferredoxin + 7 H+ | assimilatory nitrite reductase | Haloferax mediterranei | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? | |
nitrite + 6 reduced ferredoxin + 7 H+ | ferredoxin is almost as efficient as methyl viologen as electron donor | Haloferax mediterranei | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? | |
nitrite + 6 reduced ferredoxin + 7 H+ | assimilatory nitrite reductase | Haloferax mediterranei DSM 1411 | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? | |
nitrite + 6 reduced ferredoxin + 7 H+ | ferredoxin is almost as efficient as methyl viologen as electron donor | Haloferax mediterranei DSM 1411 | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? | |
nitrite + FADH2 | 50% of maximum activity compared to reduced methyl viologen as electron donor | Haloferax mediterranei | NH3 + FAD + H2O | - |
? | |
nitrite + FADH2 | 50% of maximum activity compared to reduced methyl viologen as electron donor | Haloferax mediterranei DSM 1411 | NH3 + FAD + H2O | - |
? | |
nitrite + NADPH + H+ | 10% of maximum activity compared to reduced methyl viologen as electron donor | Haloferax mediterranei | NH3 + NADP+ + H2O | - |
? | |
nitrite + reduced 2,6-dichlorophenolindophenol | less than 35% of maximum activity compared to reduced methyl viologen as electron donor | Haloferax mediterranei | NH3 + oxidized 2,6-dichlorophenolindophenol + H2O | - |
? | |
nitrite + reduced methyl viologen | ferredoxin is almost as efficient as methyl viologen as electron donor | Haloferax mediterranei | NH3 + H2O + oxidized methyl viologen | - |
? | |
nitrite + reduced methyl viologen | ferredoxin is almost as efficient as methyl viologen as electron donor | Haloferax mediterranei DSM 1411 | NH3 + H2O + oxidized methyl viologen | - |
? | |
nitrite + reduced phenazine methosulphate | less than 35% of maximum activity compared to reduced methyl viologen as electron donor | Haloferax mediterranei | NH3 + oxidized phenazine methosulphate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000 | Haloferax mediterranei |
Synonyms | Comment | Organism |
---|---|---|
assimilatory NiR | - |
Haloferax mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
when measured in the presence of 3.3 M NaCl | Haloferax mediterranei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
at 40°C | Haloferax mediterranei |
7.5 | - |
at 60°C | Haloferax mediterranei |
General Information | Comment | Organism |
---|---|---|
physiological function | assimilatory nitrite reductase | Haloferax mediterranei |