Cloned (Comment) | Organism |
---|---|
expressed as His-tagged fusion protein in Escherichia coli | Pseudomonas sp. WBC-3 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
p-benzoquinone | - |
Pseudomonas sp. WBC-3 | |
0.181 | - |
NADPH | - |
Pseudomonas sp. WBC-3 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
SDS-PAGE | Pseudomonas sp. WBC-3 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. WBC-3 | C1I202 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni2+-NTA affinity chromatography | Pseudomonas sp. WBC-3 |
Storage Stability | Organism |
---|---|
-70°C, 50% glycerol, 1 month, no loss of activity | Pseudomonas sp. WBC-3 |
0°, on ice, 12 h, fairly stable | Pseudomonas sp. WBC-3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + H+ + p-benzoquinone | - |
Pseudomonas sp. WBC-3 | NADP+ + hydroquinone | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | SDS-PAGE, 2 * 25000 Da | Pseudomonas sp. WBC-3 |
Synonyms | Comment | Organism |
---|---|---|
p-Benzoquinone reductase | - |
Pseudomonas sp. WBC-3 |
PnpB | - |
Pseudomonas sp. WBC-3 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Pseudomonas sp. WBC-3 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FMN is preferred to FAD | Pseudomonas sp. WBC-3 | |
FMN | enzyme is flavin mononucleotide-dependent, FMN is preferred to FAD | Pseudomonas sp. WBC-3 | |
NADPH | enzyme is NADPH-dependent | Pseudomonas sp. WBC-3 |
General Information | Comment | Organism |
---|---|---|
physiological function | PnpB enhances the p-nitrophenol degradation by p-nitrophenol 4-monooxygenase | Pseudomonas sp. WBC-3 |